钙网蛋白介导的凋亡在早期先兆流产过程中的作用

发布时间：2018-04-17 06:10

本文选题：钙网蛋白 + 绒毛　；参考：《中华生殖与避孕杂志》2017年11期

【摘要】：目的探讨不同表达水平的钙网蛋白(calreticulin,CRT)与先兆流产的关系。方法 (1)分别应用免疫组织化学法、Western blotting和q PCR检测早期正常妊娠(n=35,均因非意愿妊娠而要求人工流产)及早期先兆流产患者人工流产后的蜕膜和绒毛组织中CRT蛋白及mRNA的表达。(2)分别转染合成并经检测的质粒m Emerald-CRT与CRT-siRNA至BeWo细胞中,检测CRT及Caspase-3的表达。结果免疫组织化学可见CRT阳性信号定位于绒毛两层滋养层细胞的胞质和胞膜上,先兆流产组染色比正常早孕组深、面积大;q PCR与Western blotting检测CRT表达量在先兆流产组明显高于正常早孕组,先兆流产组中mRNA表达量为正常早孕组的1.2倍(P0.05)。q PCR与Western blotting检测CRT与Caspase-3表达量在质粒(m Emerald-CRT)转染组高于对照组,质粒转染组中CRT及Caspase-3的表达量分别是对照组的3.5倍与2倍(P0.05),在si-RNA组低于对照组,对照组中CRT及Caspase-3的表达量分别si-RNA组的5倍与2倍。结论早期先兆流产的发生可能与CRT在绒毛和蜕膜组织中的高表达并介导凋亡相关。
[Abstract]:Objective to investigate the relationship between calreticulin (CRT) and threatened abortion.Methods 1) Western blotting and Q PCR were used to detect CRT protein in decidua and chorionic villi of patients with early threatened abortion.And the expression of mRNA. 2) were transfected into the synthesized and detected plasmids m Emerald-CRT and CRT-siRNA to BeWo cells, respectively.The expression of CRT and Caspase-3 was detected.Results Immunohistochemistry showed that CRT positive signal was located on cytoplasm and membrane of trophoblast cells in two layers of villi. The staining of threatened abortion group was deeper than that of normal early pregnancy group.The expression of mRNA in threatened abortion group was significantly higher than that in normal early pregnancy group. The expression of mRNA in threatened abortion group was 1.2 times as high as that in normal early pregnancy group. The expression of CRT and Caspase-3 in plasmid Emerald-CRT transfection group was higher than that in control group.The expression of CRT and Caspase-3 in the plasmid transfection group was 3.5 times and 2 times of that in the control group, which was lower in the si-RNA group than in the control group. The expression of CRT and Caspase-3 in the control group was 5 and 2 times higher than that in the si-RNA group, respectively.Conclusion early threatened abortion may be related to the overexpression of CRT in chorionic villi and decidua tissues and mediated apoptosis.
【作者单位】：三峡大学第二临床医学院;肿瘤微环境与免疫治疗湖北省重点实验室;
【分类号】：R714.21

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