调节性T细胞和Notch1信号通路在原因不明复发性自然流产中的作用

发布时间：2018-04-18 01:41

本文选题：原因不明复发性自然流产(URSA) + 调节性T细胞(Treg)　；参考：《中华生殖与避孕杂志》2017年06期

【摘要】：目的探讨调节性T细胞(Treg)和Notch1信号通路在原因不明复发性自然流产(URSA)中的作用。方法流式细胞仪检测URSA患者(URSA组)及正常妊娠妇女(对照组)蜕膜CD4~+CD25~+T细胞Treg表达比例,real time RT-PCR及Western blotting检测蜕膜中CD4~+T细胞中Notch1信号通路和叉头转录因子家族3(Foxp3)表达情况。结果 URSA组CD4~+CD25~+T细胞/淋巴细胞、CD4~+Foxp3~+T细胞/淋巴细胞和CD4~+Foxp3~+T细胞/CD4~+T细胞比例均低于对照组(P0.05)。URSA组CD4~+T细胞中Notch1-Ic、RBPJκ、Foxp3 m RNA及蛋白表达均低于对照组。结论 URSA患者蜕膜CD4~+T细胞中Notch1信号通路和Foxp3表达下调,CD4~+CD25~+T细胞表达比例下降,提示URSA患者Notch1信号通路和Foxp3表达下调可能阻碍CD4~+T细胞转化为CD4~+CD25~+T细胞,进而诱发免疫排斥,诱导流产。
[Abstract]:Objective to investigate the regulatory T cells (Treg) and Notch1 signaling pathway in unexplained recurrent spontaneous abortion (URSA) in vitro. Methods flow cytometry was used to detect URSA patients (URSA group) and normal pregnant women (control group) decidual CD4~+CD25~+T cells Treg expression ratio of real time RT-PCR Western blotting, and CD4~+T in decidual detection cells in the Notch1 signaling pathway and the forkhead transcription factor family 3 (Foxp3) expression. Results CD4~+CD25~+T cells / URSA group CD4~+Foxp3~+T cells / lymphocytes, percentage of lymphocytes and CD4~+Foxp3~+T cells in /CD4~+T cells were lower than the control group (P0.05) Notch1-Ic,.URSA group CD4~+T cells RBPJ K, m RNA and protein expression of Foxp3 was lower than the control group. The down-regulation of Notch1 signaling pathway and the expression of Foxp3 in patients with URSA conclusion decidual CD4~+T cells, CD4~+CD25~+T cells decreased the proportion of patients with Notch1 suggest that URSA signaling pathway and Foxp3 Down regulation may inhibit the transformation of CD4~+T cells into CD4~+CD25~+T cells, and then induce immune rejection and induce abortion.

【作者单位】：复旦大学附属妇产科医院;
【分类号】：R714.21

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