高脂饮食诱导胰岛素抵抗对于大鼠子宫内膜的影响

发布时间：2018-04-19 09:11

本文选题：胰岛素抵抗 + 子宫内膜增殖　；参考：《现代妇产科进展》2017年06期

【摘要】：目的:建立胰岛素抵抗大鼠模型,比较胰岛素抵抗及雌激素水平增高对子宫内膜增殖的影响。方法:给予性成熟的Sprague Dawley大鼠高脂饮食以建立胰岛素抵抗大鼠模型,40周后对大鼠行去势手术后,灌胃给予大鼠17β-雌二醇4周。无胰岛素抵抗大鼠为对照组。通过测定葡萄糖耐量、胰岛素耐量、胰岛素抵抗指数、血脂等确定胰岛素抵抗。通过阴道脱落细胞巴氏染色判断雌激素的作用,HE染色检测实验组与对照组大鼠子宫内膜增殖的情况。结果:高脂饮食大鼠的葡萄糖耐量、胰岛素耐量试验曲线下面积及胰岛素抵抗指数均增加。给予雌激素后,阴道脱落细胞巴氏染色可见成熟阴道上皮细胞,HE染色可见子宫内膜厚度明显增加;与无胰岛素抵抗的大鼠相比,有胰岛素抵抗的大鼠子宫内膜出现鳞状细胞化生比例大,子宫间质中嗜酸性粒细胞增多。结论:胰岛素抵抗大鼠发生子宫内膜增殖时更易发生子宫内膜鳞状细胞化生,这一现象可能与体内高胰岛素水平有关。
[Abstract]:Aim: to establish a rat model of insulin resistance and compare the effects of insulin resistance and estrogen level on endometrial proliferation.Methods: Sprague Dawley rats were given a high fat diet for 40 weeks to establish insulin resistance model. After ovariectomy, 17 尾 -estradiol was administered intragastrically for 4 weeks.Rats with no insulin resistance served as control group.Insulin resistance was determined by measuring glucose tolerance, insulin tolerance, insulin resistance index and blood lipid.The effects of estrogen on endometrial proliferation in experimental group and control group were detected by Pap staining of vaginal exfoliated cells.Results: glucose tolerance, area under insulin tolerance test curve and insulin resistance index were increased in high fat diet rats.After estrogen administration, Pap staining of vaginal exfoliated cells showed that the thickness of endometrium was significantly increased by HE staining of mature vaginal epithelial cells, which was significantly higher than that of rats without insulin resistance.The proportion of endometrial squamous cell metaplasia and eosinophils in the endometrium of insulin resistant rats were high.Conclusion: endometrial squamous cell metaplasia is more likely to occur in insulin-resistant rats during endometrial proliferation, which may be related to high insulin level in vivo.
【作者单位】：北京大学第一医院妇产科;
【基金】：国家自然科学基金资助项目(No:81272870)
【分类号】：R737.33

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