二氧化碳气腹对大鼠卵巢功能影响的研究

发布时间：2018-04-20 06:28

  本文选题：二氧化碳气腹 + 卵巢功能　； 参考：《河北联合大学》2014年硕士论文

【摘要】：目的探讨不同时间、不同压力下二氧化碳气腹对大鼠卵巢功能的影响。 方法将10周龄健康雌性SD大鼠250只观察性周期后采用处于动情间期大鼠随即分为对照组、气腹组及无气腹组。气腹组分别于15mmHg、20mmHg压力下充入医用CO2气体1h、2h，并分别于气腹结束后0h、6h、12h、24h取下腔静脉血及双侧卵巢组织。无气腹组仅进行相关手术操作，腹腔内不充入CO2气体，取下腔静脉血及双侧卵巢组织时间同气腹组。对照组直接处死取下腔静脉血及双侧卵巢组织。双抗体夹心酶联免疫（ELISA）法测定大鼠血清FSH、LH、E2、P含量。取双侧卵巢组织，黄嘌呤氧化酶法检测超氧化物歧化酶（SOD）的活性、硫代巴比妥酸法卵巢丙二醛(MDA)，卵巢组织取做HE染色观察卵巢内细胞形态。 结果1大鼠血清内雌二醇（E2）、孕酮（P）的变化：充气1h后，气腹结束后大鼠血清中含量较对照组明显下降（p＜0.01）；气腹结束6h后血清中含量较对照组下降，且较气腹结束后0h含量下降；12h后血清中含量较气腹结束后6h下降（p＜0.01），比较对照组、0h、6h下降（p＜0.01）；气腹结束24h后较对照组、气腹结束0h、6h、12h下降（p＜0.01）；充气2h后变化同充气1h，在相同时间点的变化较充气1h下降（p＜0.01）。20mmHg压力下变化同上，相同时间点变化较15mmHg更显著（p＜0.05）；2大鼠血清FSH、LH变化相同，气腹结束后大鼠血清中气腹含量明显高于对照组（p＜0.01）；气腹结束6h后血清中含量较对照组升高，且较气腹结束后0h含量升高；12h后血清中含量较气腹结束后6h升高（p＜0.01），比较对照组、0h、6h升高（p＜0.01）；气腹结束24h后较对照组、气腹结束0h、6h、12h升高；气腹结束24h后较对照组、气腹结束0h、6h、12h升高（p＜0.01）；充气2h后变化同充气1h，在相同时间点的变化较充气1h升高（p＜0.01）；20mmHg压力下变化同上，相同时间点变化较15mmHg更显著（p＜0.05）；3大鼠卵巢组织中SOD含量：气腹结束后大鼠血清中含量较对照组明显下降（p＜0.01）；气腹结束6h后血清中含量较对照组下降，且较气腹结束后0h含量下降；12h后血清中含量较气腹结束后6h下降（p＜0.01），比较对照组、0h、6h下降（p＜0.01）；气腹结束24h后较对照组、气腹结束0h、6h、12h下降（p＜0.01）；气腹结束24h后较对照组、气腹结束0h、6h、12h下降（p＜0.01）；充气2h后变化同充气1h，在相同时间点的变化较充气1h下降（p＜0.01），20mmHg压力下变化同上，相同时间点变化较15mmHg更明显（p＜0.05）；4大鼠卵巢组织中MDA含量：气腹结束后大鼠血清中含量较对照组提高（p＜0.01）；气腹结束6h后血清中含量较对照组升高，且较气腹结束后0h含量升高；12h后血清中含量较气腹结束后6h升高（p＜0.01），比较对照组、0h、6h升高（p＜0.01）；气腹结束24h后较对照组、气腹结束0h、6h、12h升高；于0h、6h、12h、24h均升高（p＜0.01）；充气2h后变化同充气1h，在相同时间点的变化较充气1h升高（p＜0.01），20mmHg压力下变化同上，相同时间点变化较15mmHg更明显（p＜0.05）；5卵巢HE染色结果显示：气腹结束后0h可见间质细胞轻度水肿，细胞体积增大，胞浆疏松；6h可见间质细胞及部分颗粒细胞、卵泡膜细胞轻度水肿，细胞体积增大，胞浆染色变浅，血管扩张，少量炎细胞浸润；12h可见间质细胞及颗粒细胞、卵泡膜细胞水肿较前明显，细胞体积较前增大，胞浆透明，呈气球样，细胞核增大，血管扩张，，炎细胞浸润明显；24h后改变更明显。压力越大，细胞水肿越明显。 结论1大鼠血清中的E2、P在相同压力下随气腹形成时间延长及结束时间延长而下降；不同压力下随气腹形成时间延长及结束时间延长而下降，且压力越大，下降越明显；2大鼠血清中FSH、LH在相同压力下随气腹形成时间延长及结束时间延长而升高；3卵巢组织中SOD含量在相同压力下随气腹形成时间延长及结束时间延长而下降；不同压力下随气腹形成时间延长及结束时间延长而下降，且压力越大，下降越明显；MDA的含量在相同压力下随气腹形成时间延长及结束时间延长而升高；不同压力下随气腹形成时间延长及结束时间延长而升高，且压力越大，升高越明显；4相同压力下，气腹形成时间越长，卵巢组织内间质及颗粒细胞、卵泡膜细胞水肿明显，随气腹结束时间延长，变化越明显；20mmHg时上述结构变化较15mmHg压力明显；
[Abstract]:Objective to investigate the effects of carbon dioxide pneumoperitoneum on ovarian function in rats at different time and pressure.
Methods 250 healthy female SD rats of 10 weeks old were divided into control group, pneumoperitoneum group and no pneumoperitoneum group. The pneumoperitoneum group was filled with 1H and 2h of medical CO2 gas under the pressure of 15mmHg and 20mmHg, respectively. After the pneumoperitoneum, 0h, 6h, 12h, 24h took the inferior vena cava blood and bilateral ovarian tissue. No pneumoperitoneum group. The CO2 gas was not filled in the abdominal cavity, the blood of the inferior vena cava and the time of the bilateral ovarian tissue were taken with the pneumoperitoneum. The control group was directly executed to take the inferior vena cava blood and bilateral ovarian tissue. The content of FSH, LH, E2 and P in the serum of rats was measured by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). The bilateral ovarian tissue and xanthine oxidase method were measured. Superoxide dismutase (SOD) activity, thiobarbituric acid malondialdehyde (MDA), ovarian tissue HE staining were used to observe the morphology of ovarian cells.
Results the changes of serum estradiol (E2) and progesterone (P) in the 1 rats: after inflatable 1H, the serum content of rats decreased significantly after the end of pneumoperitoneum compared with the control group (P < 0.01). The content of serum in the pneumoperitoneum after 6h was lower than that of the control group, and the content of 0h decreased after the end of the pneumoperitoneum, and the serum content was lower than that of the pneumoperitoneum after the end of pneumoperitoneum (P < 0.01), and the comparison was compared with that after the end of pneumoperitoneum (P < 0.01). In the group, 0h, 6h decreased (P < 0.01); after the pneumoperitoneum ended 24h compared with the control group, the pneumoperitoneum ended with 0h, 6h, 12h (P < 0.01), the inflatable 2H was changed with the inflatable 1H, and the change in the same time point was higher than that of the inflatable 1H (P < 0.01), and the same time point was more significant than that of the inflatable 1H (< 0.05). 2 rat serum was similar, The content of pneumoperitoneum in the serum of rats after pneumoperitoneum was significantly higher than that of the control group (P < 0.01), and the content of serum in the 6h after the pneumoperitoneum was higher than that of the control group, and the content of 0h increased after the end of the pneumoperitoneum, and the content of the serum was higher than that of the control group after the end of the pneumoperitoneum (P < 0.01), compared with the control group, 0h, 6h increased (P < 0.01), and the pneumoperitoneum was compared with the control group after the pneumoperitoneum ended 24h. The abdominal end 0h, 6h, 12h increased, after the pneumoperitoneum ended 24h compared with the control group, 0h, 6h, 12h increased (P < 0.01) at the end of the pneumoperitoneum (P < 0.01); the change of inflatable 2H was higher than the inflatable 1H (P < 0.01) at the same time point (P < 0.05). The content of serum in rats after pneumoperitoneum was significantly lower than that of the control group (P < 0.01), the content of serum in the 6h after pneumoperitoneum was lower than that of the control group, and the 0h content decreased after the end of the pneumoperitoneum. After 12h, the content of serum was lower than 6H (P < 0.01) after the end of pneumoperitoneum (P < 0.01). Compared with the control group, 0h, 6h decreased (P < 0.01); after pneumoperitoneum ended 24h, the pneumoperitoneum was compared with the control group and pneumoperitoneum. The end of 0h, 6h, 12h decreased (P < 0.01); after the pneumoperitoneum ended 24h compared with the control group, the pneumoperitoneum ended 0h, 6h, 12h decreased (P < 0.01), the inflatable 2H changed with the inflatable 1H, and the change in the same time point was lower than that of the inflatable 1H (< 0.01). Content: after the end of pneumoperitoneum, the content of serum in rats increased (P < 0.01), and the content of serum in 6h after pneumoperitoneum was higher than that of control group, and the content of 0h increased after the end of pneumoperitoneum. After 12h, the content of serum was higher than that of 6h after pneumoperitoneum (P < 0.01). Compared with the control group, 0h, 6h increased (P < 0.01); after pneumoperitoneum ended 24h, the pneumoperitoneum was compared with the control group and pneumoperitoneum. At the end of 0h, 6h, and 12h, 0h, 6h, 12h, 24h increased (P < 0.01), and inflatable 1H with inflatable 2H (P < 0.01) at the same time point (P < 0.01). The same time point changes were more obvious than that of inflatable 1H (< 0.05). 5 ovary staining results showed that interstitial cells were mild after the end of the pneumoperitoneum. Edema, cell volume increased, cytoplasm loosely, 6h could be seen in interstitial cells and some granulosa cells, the follicle cells were edema, cell volume increased, cytoplasm staining became shallow, blood vessels dilated, small amount of inflammatory cells infiltrated, interstitial cells and granulosa cells were seen in 12h, the oedema of follicular cells was more obvious, cell volume was larger, cytoplasm transparent, and more transparent. Ballooning, enlarged nuclei, dilated blood vessels, inflammatory cell infiltration, and more obvious changes after 24h. The greater the pressure, the more obvious the cell edema.
Conclusion the E2 and P in the serum of 1 rats decreased with the prolongation of the time of pneumoperitoneum formation and the extension of the end time. Under the different pressure, the longer the formation time and the end time of the pneumoperitoneum, the greater the pressure, the more obvious. The FSH and LH in the serum of 2 rats were prolonged with the prolongation of the pneumoperitoneum and the end time under the same pressure. The content of SOD in the ovarian tissue decreased with the prolongation of the time of pneumoperitoneum and the extension of the end time under the same pressure. The greater the pressure of the pneumoperitoneum and the longer the end time, the decrease was more obvious under the pressure of the pneumoperitoneum. The content of MDA was prolonged with the time of the pneumoperitoneum and the end time under the same pressure. Under the same pressure, the longer the formation time of the pneumoperitoneum, the greater the formation time of the pneumoperitoneum, the edema of the ovarian tissue and granulosa cells, the follicle membrane cells in the ovarian tissue, the more obvious the change with the end of the pneumoperitoneum at the same time, and the above structure at 20mmHg at 20mmHg. The change is more obvious than that of 15mmHg.

【学位授予单位】：河北联合大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R71

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