PLGA生物可降解膜用于预防宫腔粘连的初步研究

发布时间：2018-04-20 09:13

本文选题：PLGA + 宫腔粘连　；参考：《重庆医科大学学报》2017年04期

【摘要】：目的:研究聚乳酸-羟基乙酸共聚物[poly(lactic-co-glycolic acid),PLGA]膜作为生物可降解材料用于预防宫腔粘连的可行性初步研究。方法:制备不同单体比例的PLGA膜,分别于模拟宫腔液以及昆明小鼠宫腔中进行体外和体内降解实验。将30只昆明小鼠随机分为假手术组、宫腔粘连组、PLGA膜组,每组各10只,运用Masson染色和Western blot观察各组昆明小鼠子宫内膜纤维化程度和相关蛋白的表达。结果:不同单体比例的PLGA膜体外降解时间不同,PLGA膜(50∶50)的降解速度最快,在4周时质量损失达98.5%;PLGA膜(90∶10)降解速度最慢,在12周时其质量损失仅为7.8%;PLGA(75∶25)降解速度介于前两者之间,于12周时质量损失达95.7%。不同单体比例的PLGA膜体内降解时间不同,PLGA膜(50∶50)7 d后在宫腔内完全降解,而PLGA膜(75∶25)、PLGA膜(90∶10)仍然滞留于宫腔中。与假手术组相比,PLGA膜组(75∶25)增加了昆明小鼠子宫内膜纤维化面积(P=0.000)以及相关蛋白的表达(P0.01);与宫腔粘连组相比,PLGA膜组(75∶25)子宫内膜纤维化面积以及相关蛋白的表达无明显差异(P0.05)。结论:PLGA生物可降解材料用于预防宫腔粘连,具有宫腔内降解速度可调节、不增加宫腔粘连组子宫内膜纤维化程度的特点,在预防宫腔粘连方面具有潜在的应用价值。
[Abstract]:Aim: to study the feasibility of poly(lactic-co-glycolic acidoglycolic acid (poly(lactic-co-glycolic) membrane as biodegradable material for preventing intrauterine adhesion. Methods: PLGA membranes with different monomer ratios were prepared and degraded in vitro and in vivo in simulated uterine fluid and Kunming mouse uterine cavity respectively. Thirty Kunming mice were randomly divided into sham operation group and intrauterine adhesion group with 10 mice in each group. The degree of endometrial fibrosis and the expression of related proteins in Kunming mice were observed by Masson staining and Western blot. Results: the degradation rate of PLGA membrane with different monomer ratio in vitro was the fastest, and the mass loss reached 98.55% at 4 weeks. The degradation rate of PLGA 75: 25 was between the former two at 12 weeks, and the mass loss reached 95.7at 12 weeks. The degradation time of PLGA membrane with different monomer ratio was 50: 507 days later, and PLGA membrane 75: 25 / PLGA membrane 90: 10 remained in uterine cavity. Compared with sham operation group, PLGA membrane group (75: 25) increased the area of endometrial fibrosis in Kunming mice (P0. 000) and the expression of related protein (P0. 01); compared with the group of intrauterine adhesion, PLGA membrane group (75: 25) increased the area of endometrial fibrosis and related proteins. There was no significant difference in expression of P0.05. Conclusion the biodegradable material of% PLGA can be used to prevent intrauterine adhesion, which has the characteristics of adjustable degradation rate in uterine cavity and no increase in the degree of endometrial fibrosis in the group of intrauterine adhesions. It has potential application value in the prevention of intrauterine adhesions.
【作者单位】：重庆医科大学附属第二医院妇产科;
【分类号】：R711.74

【相似文献】