负载抗sca-1抗体和碱性成纤维细胞生长因子的脱细胞膀胱基质的生物性能研究

发布时间：2018-04-22 08:20

  本文选题：抗sca-抗体 + 碱性成纤维细胞生长因子　； 参考：《第三军医大学学报》2017年22期

【摘要】：目的观察负载抗sca-1抗体(anti-sca-1)和碱性成纤维细胞生长因子(basic fibroblast growth factor,b FGF)-脱细胞膀胱基质(urinary bladder matrix,UBM)生物支架的生物学性能,探讨其作为新型盆底修复材料的可能性。方法通过双特异性化学交联试剂4-(N-马来酰亚胺甲基)环己烷-1-羧酸磺酸基琥珀酰亚胺酯钠盐(sulfosuccinimidyl 4-[N-maleimidomethyl]cyclohexane-1-carboxylate,sulfosmcc)将anti-sca-1和b FGF特异性结合到UBM上,得到anti-sca-1/b FGF-UBM生物支架。通过扫描电子显微镜观察生物支架表面结构,CCK-8(cell counting kit-8)间接检测体外富集sca-1阳性细胞情况,免疫荧光观察支架诱导小鼠骨髓间充质干细胞(mouse mesenchymal stem cells,m MSCs)分化成熟情况。结果扫描电子显微镜显示anti-sca-1和b FGF能特异性的结合到UBM上。anti-sca-1和b FGF组荧光强度明显高于对照组(P0.05)。anti-sca-1/b FGF-UBM生物支架富集的sca-1阳性细胞明显较对照组多(P0.05)。免疫荧光观察结果提示anti-sca-1/b FGF-UBM生物支架可以促进m MSCs向平滑肌细胞分化。结论 anti-sca-1/b FGF-UBM生物支架显示出了良好的生物性能,既能富集sca-1阳性细胞,又能进一步促进细胞分化成熟。
[Abstract]:Objective to investigate the biological properties of scaffolds loaded with anti-sca-1 (anti-sca-1) and basic fibroblast growth factor-basic fibroblast growth factor-acellular bladder matrix (bladder), and to explore the possibility of using the scaffold as a new pelvic floor repair material. Methods anti-sca-1 and b FGF were specifically conjugated to UBM by bispecific chemical crosslinking reagent 4-N- maleimide methyl) cyclohexane-1-carboxylic acid sulfonyl succinimidyl 4- [N-maleimidomethyl] cyclohexane-1-carboxylate sulfoxylsuccinyl 4- [N-maleimidomethyl] cyclohexane-1-carboxylatesulfosmcc. the anti-sca-1/b FGF-UBM scaffolds were obtained. The surface structure of CCK-8 cell counting kit-8 was observed by scanning electron microscope (SEM). The enrichment of sca-1 positive cells was detected indirectly in vitro. The differentiation and maturation of mesenchymal stem cells were observed by immunofluorescence in mice bone marrow mesenchymal stem cells (MSCs) induced by the scaffold. Results scanning electron microscopy showed that the fluorescence intensity of anti-sca-1 and b FGF binding to UBM. Anti-sca-1 and b FGF was significantly higher than that of control group (P0.05, anti-sca-1 / 1 / b FGF-UBM bioscaffold), and the number of sca-1 positive cells was significantly higher than that of control group. Immunofluorescence observation showed that anti-sca-1/b FGF-UBM scaffold could promote the differentiation of m MSCs into smooth muscle cells. Conclusion anti-sca-1/b FGF-UBM scaffolds have good biological properties, which can not only enrich sca-1 positive cells, but also further promote cell differentiation and maturation.
【作者单位】： 第三军医大学西南医院妇产科;
【基金】：国家自然科学基金面上项目(81471443)~~
【分类号】：R318.08;R711.2
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本文编号：1786347