骨髓间充质干细胞对顺铂所致的大鼠窦前卵泡损伤的保护作用

发布时间：2018-04-25 02:11

本文选题：骨髓间充质干细胞 + 顺铂　；参考：《实用妇产科杂志》2015年09期

【摘要】：目的:观察大鼠骨髓间充质干细胞(MSCs)对顺铂(DDP)所致的体外培养大鼠窦前卵泡损伤的保护作用。方法:实验分为3组,每组为30个窦前卵泡。实验组:取第3代MSCs,贴壁生长后,将大鼠窦前卵泡移入培养板内共培养24小时后换液,加入1μg/ml DDP,作用24小时后换新鲜基础培养液;实验对照组:未行MSCs共培养,直接将大鼠窦前卵泡移入培养液中培养,后面方法同实验组;空白对照组:大鼠窦前卵泡移入培养液中培养,不加入DDP。光镜下观察3组窦前卵泡的形态学变化,采用化学发光法分别检测加药后第3、5、7天各组培养液中雌二醇(E2)的浓度。结果:1实验组窦前卵泡第3、5天均存活,第7天仅少数窦前卵泡变黑,但卵母细胞清晰可见;实验对照组窦前卵泡第3天出现退化现象,第7天则完全退化;空白对照组窦前卵泡第3天形态尚规整,但第7天大多数卵泡出现退化现象。2实验组中E2含量随着时间的变化逐渐升高(P0.05);空白对照组E2含量随着时间的变化逐渐下降(P0.05);实验对照组E2含量最低,变化不大,3个时间点比较,差异无统计学意义(P0.05)。3组间大鼠窦前卵泡培养液中E2含量间有主效应(P0.05),时间和组别之间存在交互效应(P0.05)。结论:MSCs对DDP引起的窦前卵泡的损伤可能有保护作用,可恢复并促进窦前卵泡的内分泌功能。
[Abstract]:Aim: to observe the protective effect of MSCs on rat preantral follicle injury induced by cisplatin (DDP) in vitro. Methods: the experiment was divided into 3 groups, 30 preantral follicles in each group. Experimental group: after the third passage of MSCs, the rat preantral follicles were transplanted into the culture plate for 24 hours, and then added 1 渭 g/ml DDP for 24 hours to replace the fresh basic culture medium, while the experimental control group: no MSCs co-culture was carried out. The rat preantral follicles were cultured in culture medium, the latter method was the same as the experimental group, and the blank control group: the rat preantral follicles were cultured in the culture medium without adding DDP. The morphological changes of preantral follicles in three groups were observed under light microscope. The concentration of estradiol E _ 2 in the culture medium of each group was detected by chemiluminescence method. Results in the experimental group, preantral follicles survived on the 3rd day, only a few follicles became black on the 7th day, but the oocytes were clearly visible, while in the control group, the preantral follicles degenerated on the 3rd day and completely degenerated on the 7th day. In the blank control group, the shape of preantral follicle was regular on the 3rd day. On the 7th day, the E2 content in most follicles of the experimental group increased gradually with the change of time, while that in the blank control group decreased gradually with the change of time, and that in the control group was the lowest. There was no significant difference in E _ 2 content in preantral follicle culture of rats at three time points. There was a major effect between E _ 2 and P _ (0.05), and there was interaction between time and group (P _ (0.05)). Conclusion DDP may have protective effect on preantral follicle injury and can restore and promote the endocrine function of preantral follicle.
【作者单位】：昆明医科大学附属第三医院;云南省肿瘤医院;南方医科大学珠江医院;
【分类号】：R711.75

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