C-myc和cyclinD1在子宫内膜异位症发病机制中的研究

发布时间：2018-04-25 02:12

  本文选题：子宫内膜异位症 + 雌激素　； 参考：《福建医科大学》2014年硕士论文

【摘要】：【目的】 检测子宫内膜异位症患者中雌激素靶基因c-myc和cyclinD1的表达，探讨它们之间是否存在相关性；分析子宫内膜异位症患者不同分期的c-myc和cyclinD1的表达是否存在差异；探讨病例组中c-myc与cyclinD1的表达与患者CA125之间是否存在相关性。 【方法】 依据诊断标准入选，，从2012年07月到2013年12月泉州市妇幼保健院开腹或腹腔镜手术治疗的EMS患者30例为病例组；同期因单纯输卵管病变行手术治疗的患者30例为对照组。采用免疫组化法和real time PCR检测病例组的在位子宫内膜和腹膜异位病灶组织及对照组的子宫内膜和正常腹膜组织的c-myc和cyclinD1的表达情况。 【结果】 1、一般临床资料比较：病例组和对照组的年龄无统计学差异(P0.05)。 2、C-myc和cyclinD1在病例组的在位子宫内膜和腹膜异位病灶组织及对照组的子宫内膜和正常腹膜组织中均有表达。与对照组相比，病例组的在位子宫内膜和腹膜异位病灶组织c-myc和cyclinD1呈过度表达。 3、不同分期的子宫内膜异位症患者的在位子宫内膜和腹膜异位病灶组织的c-myc和cyclinD1的表达无统计学差异(P0.05)。 4、在病例组c-myc与cyclinD1的表达呈显著正相关。 5、在病例组中c-myc与cyclinD1的表达与患者CA125无相关性。 【结论】 1、C-myc和cyclinD1在病例组的在位子宫内膜和腹膜异位病灶组织的表达明显高于对照组在位子宫内膜和腹膜组织。 2、C-myc和cyclinD1与子宫内膜异位症有关，异常表达的c-myc与cyclinD1协同加速周期细胞G1-S进展，促进细胞增殖。 3、在病例组中c-myc和cyclinD1的表达与患者的分期及CA125无相关性。
[Abstract]:[purpose] To detect the expression of estrogen target gene (c-myc) and estrogen target gene (cyclinD1) in patients with endometriosis, to explore the correlation between them, and to analyze the difference of c-myc and cyclinD1 expression in different stages of endometriosis. To investigate the correlation between the expression of c-myc and cyclinD1 and CA125 in patients. [methods] According to the diagnostic criteria, 30 patients with EMS treated by laparoscopy or laparotomy from July 2012 to December 2013 in Quanzhou Maternal and Child Health Hospital were selected as the case group, and 30 patients as the control group who underwent surgical treatment for simple fallopian tube disease in the same period. Immunohistochemical method and real time PCR were used to detect the expression of c-myc and cyclinD1 in eutopic endometrium and peritoneal ectopic lesions and the endometrium and normal peritoneum of the control group. [results] 1. Comparison of general clinical data: there was no significant difference in age between the case group and the control group (P 0.05). 2C-myc and cyclinD1 were expressed in eutopic endometrium and peritoneal ectopic lesions, and in endometrium and normal peritoneum of control group. Compared with the control group, c-myc and cyclinD1 were overexpressed in eutopic endometrium and ectopic peritoneal lesions. 3There was no significant difference in the expression of c-myc and cyclinD1 between eutopic endometrium and peritoneal ectopic lesions in patients with different stages of endometriosis (P 0.05). 4. There was a significant positive correlation between the expression of c-myc and cyclinD1 in the case group. 5. There was no correlation between the expression of c-myc and cyclinD1 and CA125. [conclusion] 1the expression of C-myc and cyclinD1 in eutopic endometrium and peritoneal ectopic lesions in the case group was significantly higher than that in the eutopic endometrium and peritoneal tissue of the control group. 2C-myc and cyclinD1 are associated with endometriosis. Abnormal expression of c-myc and cyclinD1 can accelerate the progression of G1-S cells and promote cell proliferation. 3. There was no correlation between the expression of c-myc and cyclinD1 and the stage and CA125 of the patients.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R711.71

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