孕激素对重度子痫前期TLR4信号通路的调控作用

发布时间：2018-04-25 19:44

  本文选题：孕激素 + 重度子痫前期　； 参考：《实用妇产科杂志》2015年11期

【摘要】：目的:研究孕激素在重度子痫前期孕妇外周血血浆中的水平及其在重度子痫前期发病机制Toll样受体4(TLR4)信号通路中的作用。方法:选择重度子痫前期孕妇30例(重度子痫前期组)和正常孕妇30例(正常对照组),采用稀释定量法分别测定其血浆孕激素水平;原代培养重度子痫前期组孕妇外周血单个核细胞(PBMC),以不同浓度(0、10-8mol/L、10-6mol/L、10-4mol/L)孕激素(孕酮)刺激,实时定量聚合酶联反应检测TLR4、髓样分化因子88(My D88)、核转录因子-κB(NF-κB)mRNA的表达;酶联免疫吸附试验检测肿瘤坏死因子-α(TNF-α)及白介素-6(IL-6)蛋白的表达。结果:重度子痫前期组孕妇血浆孕激素水平明显低于正常对照组(P0.05)。随着孕激素浓度的增加,重度子痫前期组孕妇TLR4、My D88、NF-κB mRNA的相对表达量逐渐减少,4组组间两两比较,差异均有统计学意义(P0.01);TNF-α及IL-6蛋白的表达均减少,4组组间两两比较,差异均有统计学意义(P0.01)。结论:孕激素水平在重度子痫前期孕妇外周血浆中明显降低,孕激素可在重度子痫前期发病机制TLR4信号通路中发挥抑制作用,从而对机体产生保护作用。
[Abstract]:Objective: To study the role of progestin in peripheral blood plasma in pregnant women with severe preeclampsia and its role in the Toll like receptor 4 (TLR4) signaling pathway in the pathogenesis of severe preeclampsia. Methods: 30 cases of severe preeclampsia (severe preeclampsia) and 30 normal pregnant women (normal control group) were selected and measured by dilution quantitative method respectively. Plasma progesterone level; primary cultured preeclampsia pregnant women peripheral blood mononuclear cells (PBMC), with different concentrations (0,10-8mol/L, 10-6mol/L, 10-4mol/L) progesterone (progesterone) stimulation, real-time quantitative polymerase chain reaction to detect TLR4, myeloid differentiation factor 88 (My D88), nuclear factor kappa B (NF- kappa B) mRNA expression; enzyme linked immunosorbent assay The expression of tumor necrosis factor - alpha (TNF- - alpha) and interleukin -6 (IL-6) protein was detected. Results: the plasma progesterone level of pregnant women with severe preeclampsia was significantly lower than that of the normal control group (P0.05). With the increase of the concentration of progestin, the relative expression of TLR4, My D88, NF- kappa B mRNA in pregnant women with severe preeclampsia gradually decreased, and the difference between the 4 groups was 22, and the difference was poor. The difference was statistically significant (P0.01), the expression of TNF- alpha and IL-6 protein decreased, and the difference between the 4 groups was statistically significant (P0.01). Conclusion: progestin levels in the peripheral plasma of pregnant women with severe preeclampsia were significantly decreased, progestin could play an inhibitory role in the pathogenesis of severe pre eclampsia TLR4 signaling pathway. The body has a protective effect.

【作者单位】： 华中科技大学同济医学院附属协和医院;
【分类号】：R714.244
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本文编号：1802690