氯高铁血红素对妊娠期高血压大鼠的治疗及机制

发布时间：2018-04-26 09:24

本文选题：氯高铁血红素 + 血红素氧合酶　；参考：《南方医科大学学报》2015年04期

【摘要】：目的初步探讨血红素氧合酶诱导剂氯高铁血红素(Hemin)对妊娠期高血压(HDCP)大鼠的治疗作用及可能调控机制。方法将18只受孕SD大鼠于妊娠第12天随机分为3组(6只/组):HDCP模型组、Hemin干预组、正常妊娠组。HDCP模型组和Hemin干预组于妊娠第14天起连续7 d予亚硝基左旋精氨酸甲酯(80 mg/kg)灌胃建立HDCP模型,正常妊娠组予等量生理盐水灌胃处理,Hemin干预组于妊娠第16天起每日下午腹腔注射Hemin(30 mg/kg)。用分光光度法测定各组胎盘组织血红素氧合酶(HO)的活性和碳氧血红蛋白(COHb)水平,ELSIA测定各组胎盘组织匀浆上清液可溶性血管内皮生长因子受体-1(s FIt-1)、血管内皮生长因子(VEGF)水平。结果妊娠第20天,HDCP模型组孕鼠血压和24 h尿蛋白明显高于正常妊娠组和Hemin干预组(P0.05),而HO活性和COHb含量明显低于正常妊娠组和Hemin干预组(P0.05),Hemin干预组血压及24 h尿蛋白高于正常组(P0.05),而HO活性和COHb含量较正常组低(P0.05);HDCP模型组孕鼠胎盘组织s FIt-1水平明显高于正常妊娠组和Hemin干预组(P0.05),而胎盘组织中VEGF水平明显低于正常妊娠组和Hemin干预组(P0.05),Hemin干预组孕鼠胎盘组织s FIt-1水平高于正常组水平(P0.05),而VEGF水平低于正常组水平(P0.05)。结论 Hemin能够降低妊娠期高血压孕鼠的血压及尿蛋白,其可能机制是通过上调胎盘组织中HO的活性,增加代谢产物CO,降低胎盘组织中s FIt-1,并升高VEGF水平来发挥调控作用的。
[Abstract]:Objective to investigate the therapeutic effect of heme oxygenase inducer (Heminin) on hypertensive pregnant rats (HDCPs) and its possible regulatory mechanism. Methods on the 12th day of gestation, 18 pregnant SD rats were randomly divided into 3 groups (n = 6) and one group (n = 6). The HDCP model was established in normal pregnancy group, HDCP model group and Hemin intervention group by intragastric administration of nitro-L-arginine methyl ester 80 mg / kg for 7 days from the 14th day of gestation. The normal pregnancy group was treated with the same amount of normal saline intragastric administration of Hemin on the 16th day of pregnancy, Hemin(30 mg / kg / kg was injected intraperitoneally every afternoon from the 16th day of gestation. The activity of heme oxygenase (HOH) and the level of carboxyhemoglobin (COHb) in placenta tissue of each group were determined by spectrophotometry. The levels of soluble vascular endothelial growth factor receptor -1s FIt-1 and vascular endothelial growth factor (VEGF) in the supernatant of placenta homogenate of each group were determined by ELSIA. Results on the 20th day of pregnancy, the blood pressure and 24 h urine protein of pregnant rats in HDCP model group were significantly higher than those in normal pregnancy group and Hemin intervention group, while Ho activity and COHb content were significantly lower than those in normal pregnancy group and Hemin intervention group. The activity of Ho and the content of COHb in placenta of pregnant rats were significantly higher than those of normal pregnancy group and Hemin intervention group, while the level of VEGF in placenta was significantly lower than that in normal pregnancy group and Hemin intervention group. The level of s FIt-1 in placenta of pregnant rats in group P0.05 was higher than that in normal group, while the level of VEGF was lower than that of P0.05 in normal group. Conclusion Hemin can decrease the blood pressure and urine protein in pregnant rats with hypertension complicating pregnancy. The possible mechanism is to increase the activity of Ho in placenta, increase the metabolite COand decrease the level of sFIt-1 in placenta and increase the level of VEGF.
【作者单位】：中南大学湘雅三医院妇产科;
【基金】：湖南省自然科学基金(2015JJ4050)
【分类号】：R714.246

【参考文献】