孕妇外周血胎儿有核红细胞计数变化及其在不良妊娠结局预测中的应用

发布时间：2018-04-26 15:35

本文选题：有核红细胞 + 胎儿有核红细胞　；参考：《山东医药》2015年43期

【摘要】：目的观察孕妇外周血胎儿有核红细胞计数的变化,探讨其在妊娠不良结局预测中的应用价值。方法收集87例孕12~18周孕妇的外周血,密度梯度离心法分离单个核细胞,采用PE-GPA/FITC-CD71单克隆抗体双色标记胎儿有核红细胞后进行流式细胞分析,追踪妊娠结局并进行分析。结果妊娠结局正常者外周血中胎儿有核红细胞百分比为0.89%±0.81%,妊娠结局不良者为3.25%±2.98%,两者相比,P0.05。以胎儿有核红细胞百分比3.79%作为截断值,预测妊娠不良结局的敏感度为76.9%、特异度为64.3%。结论与妊娠结局正常的孕妇相比,妊娠结局不良孕妇孕12~18周时外周血胎儿有核红细胞增多;对孕12~18周孕妇外周血胎儿有核红细胞进行计数有助于不良妊娠结局的预测,其敏感度及特异度均较高。
[Abstract]:Objective to observe the changes of fetal nucleated red blood cells (PBMC) in maternal peripheral blood and to explore its value in predicting the adverse outcome of pregnancy. Methods Mononuclear cells were isolated from the peripheral blood of 87 pregnant women at 12 ~ 18 weeks of gestation by density gradient centrifugation. The fetal nucleated red blood cells were labeled with PE-GPA/FITC-CD71 monoclonal antibody and analyzed by flow cytometry. The pregnancy outcome was followed up and analyzed. Results the percentage of fetal nucleated erythrocytes in peripheral blood of normal pregnant women was 0.89% 卤0.81%, and that of poor pregnancy outcome was 3.25% 卤2.98, compared with that of P0.05. When the percentage of fetal nucleated red blood cells was 3.79%, the sensitivity and specificity of predicting the adverse outcome of pregnancy were 76.9 and 64.3 respectively. Conclusion compared with pregnant women with normal pregnancy outcome, peripheral blood fetal nucleated red blood cells (PBMC) of pregnant women with poor pregnancy outcome increased at 12w / 18 weeks, and counting of peripheral blood fetal nucleated red blood cells (PBMC) was helpful to predict adverse pregnancy outcome. Its sensitivity and specificity are higher.
【作者单位】：武汉市妇女儿童医疗保健中心;安徽医科大学第二附属医院;武汉大学中南医院;
【基金】：湖北省武汉市卫生和计划生育委员会2014年立项课题
【分类号】：R714.5

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