卵丘颗粒细胞SIRT1表达对卵胞浆内单精子显微注射助孕结局的影响

发布时间：2018-04-29 21:49

  本文选题：卵胞浆内单精子注射 + 卵丘颗粒细胞　； 参考：《福建医药杂志》2016年06期

【摘要】：目的探讨卵丘颗粒细胞SIRT1表达对ICSI助孕结局的影响。方法收集120例因男方因素在我院辅助生殖技术研究室行ICSI助孕的女性患者卵丘颗粒细胞,采用免疫组化法检测SIRT1蛋白。比较分析ICSI妊娠与非妊娠组SIRT1表达率,并分析其与ICSI实验室各项指标如穿卵泡数、获卵数、获卵率,受精数、受精率,卵裂数、卵裂率,优质胚胎数、囊胚形成数及囊胚冷冻数之间关系。结果妊娠与未妊娠组SIRT1表达率差异有统计学意义(P0.05)。SIRT1表达率与穿卵泡数、Ⅲ级OCCs数、ICSI卵子数、受精数、卵裂数、平均优质胚胎数、平均囊胚数、优质囊胚数、平均冷冻胚胎数间均有显著相关性(P0.01)。结论 SIRT1在卵丘颗粒细胞表达可能影响卵母细胞成熟及卵子发育能力,进而与ICSI助孕结局有关。
[Abstract]:Objective to investigate the effect of SIRT1 expression in cumulus granulosa cells on the outcome of ICSI assisted pregnancy. Methods the cumulus granulosa cells were collected from 120 female women who were pregnant with ICSI because of male factors in the assisted reproductive technology laboratory of our hospital. The SIRT1 protein was detected by immunohistochemical method. The expression rate of SIRT1 in ICSI pregnancy group and non-pregnancy group was compared and compared with ICSI laboratory indexes such as the number of follicles pierced, the rate of obtaining eggs, the number of fertilization, the rate of fertilization, the number of cleavage, the rate of cleavage and the number of high-quality embryos. The relationship between the number of blastocysts and the number of frozen blastocysts. Results there were significant differences in SIRT1 expression rate between pregnant and non-pregnant women (P 0.05). SIRT1 expression rate was significantly higher than that in unpregnant women (P 0.05). The number of follicles, the number of SIRT1 eggs, fertilization, cleavage, average number of high quality embryos, average number of blastocysts, number of high quality blastocysts, and the number of high quality blastocysts were significantly different between pregnant and non-pregnant women. There was a significant correlation between the average number of frozen embryos and that of P0.01. Conclusion the expression of SIRT1 in cumulus granulosa cells may affect oocyte maturation and oocyte development, which may be related to the outcome of ICSI assisted pregnancy.
【作者单位】： 福建医科大学附属福建省妇幼保健院检验科;福建省妇幼保健院辅助生殖技术研究室;福建医科大学免疫学系;
【基金】：福建省妇幼保健院院内科研课题(妇保院研13-09)
【分类号】：R714.8
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本文编号：1821717