葡萄糖转运蛋白4在宫内发育迟缓大鼠骨骼肌中的表达和转位

发布时间：2018-05-01 14:25

本文选题：宫内发育迟缓 + 胰岛素抵抗　；参考：《中国现代医学杂志》2015年09期

【摘要】：目的研究宫内发育迟缓(IUGR)大鼠骨骼肌胰岛素刺激下葡萄糖转运蛋白4(GLUT4)表达和转位的变化,探讨IUGR个体发生胰岛素抵抗的分子机制。方法采用孕期全程低蛋白饮食法建立大鼠IUGR模型,检测18月龄IUGR子鼠骨骼肌胰岛素刺激下的2-D-3H葡萄糖的摄取,同时采用实时荧光定量RT-PCR和Western blot方法检测胰岛素受体(IR)、胰岛素受体底物1(IRS-1)及葡萄糖转运蛋白4(GLUT4)的m RNA和蛋白表达;用Western blot方法分别检测胰岛素刺激前后骨骼肌总/膜GLUT4的蛋白表达变化。结果 1与对照组相比,18月龄IUGR组大鼠骨骼肌胰岛素刺激后葡萄糖摄取率显著降低(P=0.035);2IR和IRS-1 m RNA和蛋白表达在两组大鼠骨骼肌中差异无统计学意义(P0.05),但GLUT4 m RNA表达在IUGR组大鼠骨骼肌中显著下降(P=0.031);3基础状态时,两组大鼠骨骼肌GLUT4总蛋白表达无明显差异(P=0.967);IUGR组GLUT4膜蛋白表达略降低,但未及统计学意义(P=0.072);经胰岛素刺激后,两组大鼠骨骼肌与各自基础状态相比,GLUT4总蛋白变化不明显,GLUT4膜蛋白表达均较各自的基础状态显著增加,但IUGR大鼠骨骼肌GLUT4膜蛋白的上升程度显著低于对照组(P=0.001)。结论 IUGR老龄大鼠骨骼肌中GLUT4 m RNA表达降低以及胰岛素刺激下的GLUT4转位下降,可能降低对胰岛素的反应性,从而减少葡萄糖摄取,促进胰岛素抵抗发生。
[Abstract]:Objective to investigate the expression and translocation of glucose transporter 4 (GLUT4) in skeletal muscle of rats with intrauterine growth retardation (IUGR), and to explore the molecular mechanism of insulin resistance in IUGR individuals. Methods the IUGR model of rats was established by low-protein diet during pregnancy, and the 2-D-3H glucose uptake in skeletal muscle of 18 month old IUGR offspring was measured by insulin stimulation. Meanwhile, real-time quantitative RT-PCR and Western blot were used to detect the expression of m RNA and protein in insulin receptor irons, insulin receptor substrates 1 (IRS-1) and glucose transporter 4 (GLUT4). The protein expression of total / membrane GLUT4 in skeletal muscle was detected by Western blot before and after insulin stimulation. Results 1 compared with the control group, the glucose uptake rate of skeletal muscle in 18-month-old IUGR group was significantly lower than that in the control group after insulin stimulation. There was no significant difference in the expression of P0.035 IR and IRS-1 m RNA and protein between the two groups, but the expression of GLUT4 m RNA in the skeletal muscle of the two groups was not significantly different from that in the control group. In the IUGR group, the skeletal muscle decreased significantly in the basic state of P0. 031. There was no significant difference between the two groups in the expression of total GLUT4 protein in skeletal muscle. The expression of GLUT4 membrane protein in the group of GLUT4 0.967 was slightly decreased, but there was no significant difference between the two groups in the expression of GLUT4 membrane protein. The expression of GLUT4 membrane protein in skeletal muscle of the two groups was significantly higher than that of the control group, but the increase of GLUT4 membrane protein in the skeletal muscle of IUGR rats was significantly lower than that in the control group (P 0.001). Conclusion the decrease of GLUT4 m RNA expression in skeletal muscle of aged IUGR rats and the decrease of GLUT4 translocation induced by insulin may decrease the response to insulin, thereby reducing glucose uptake and promoting insulin resistance.
【作者单位】：南京市妇幼保健院检验科;
【基金】：南京市医学重点科技发展项目(No:ZKX09013) 国家自然科学基金项目(No:81300654)
【分类号】：R714.5

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