子宫腔粘连患者子宫内膜组织中ER、PR的表达及意义

发布时间：2018-05-03 12:55

本文选题：子宫腔粘连 + ER　；参考：《临床与实验病理学杂志》2015年04期

【摘要】：目的探讨雌激素受体(ER)、孕激素受体(PR)在子宫腔粘连(intrauterine adheious,IUA)患者子宫内膜组织中的表达及其临床意义。方法采用免疫组化Max Vision两步法和实时荧光定量PCR(qRT-PCR)技术检测IUA组(研究组)和非IUA组(对照组)子宫内膜组织中ER、PR的表达水平。结果免疫组化检测显示ER蛋白在研究组(3.52±0.71)和对照组(2.75±1.00)中的表达差异有统计学意义(P=0.01);qRT-PCR法检测显示ER mRNA在研究组(1.59±0.26)和对照组(1.00±0.19)中的表达差异有统计学意义(P=0.00)。免疫组化检测显示PR蛋白在研究组(3.26±0.70)和对照组(3.58±0.28)中的表达差异无统计学意义(P=0.12);qRT-PCR法检测显示PR mRNA在研究组(1.15±0.21)和对照组(1.00±0.31)中的表达差异无统计学意义(P=0.21)。免疫组化检测显示ER、PR蛋白分别在子宫内膜腺体和子宫内膜间质中均有表达,差异有统计学意义(χ2=5.797,P=0.016;χ2=4.857,P=0.027)。结论子宫内膜组织中ER表达研究组高于对照组,PR在两组之间的表达无差异;ER、PR蛋白在两组子宫内膜腺体中表达较多,在子宫内膜间质中表达较少甚至不表达;ER、PR表达的特点为IUA患者临床使用雌、孕激素治疗提供一定的理论参考。
[Abstract]:Objective to investigate the expression and clinical significance of estrogen receptor ERA and progesterone receptor PRA in endometrial tissue of patients with intrauterine adhesion (IUAA). Methods the expression of ERPR in endometrium of IUA group (study group) and non- group (control group) was detected by immunohistochemical Max Vision two-step method and real-time fluorescence quantitative PCRQ RT-PCR technique. Results the expression of ER protein in the study group (3.52 卤0.71) was significantly different from that in the control group (2.75 卤1.00). The expression of ER mRNA in the study group (1.59 卤0.26) and the control group (1.00 卤0.19) was significantly different by RT-PCR. The expression of PR protein in the study group (3.26 卤0.70) and the control group (3.58 卤0.28) was not significantly different from that in the control group (P < 0.01). The expression of PR mRNA in the study group (1.15 卤0.21) and the control group (1.00 卤0.31) had no significant difference (P0.21). The expression of ERP protein in endometrial glands and endometrial stroma was detected by immunohistochemistry, and the difference was statistically significant (蠂 2 / 5.797 / P ~ (0.016); 蠂 ~ (2) = 4.857 / P ~ (0.027). Conclusion the expression of ER in endometrial tissue in study group is higher than that in control group. There is no difference in the expression of ER and PR between the two groups. The characteristics of low or no expression of ERP PR in the stroma of endometrium provide a theoretical reference for the clinical use of estrogen and progesterone in patients with IUA.
【作者单位】：安徽医科大学第二附属医院妇产科;
【基金】：国家自然科学基金青年科学基金(81100412)
【分类号】：R711.74

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