血清多肽谱检测对子宫内膜癌早期诊断价值

发布时间：2018-05-06 04:16

  本文选题：子宫内膜肿瘤 + 飞行时间质谱　； 参考：《中华肿瘤防治杂志》2015年11期

【摘要】：目的研究子宫内膜癌患者血清多肽谱的变化,探讨子宫内膜癌早期诊断相关的差异蛋白。方法选取2008-05-01-2011-05-31浙江省肿瘤医院收治的子宫内膜癌患者134例、子宫体良性病变患者103例和健康女性志愿者95名作为研究对象。将研究对象分为测试组(45例Ⅰ期子宫内膜癌患者和45名健康女性)、验证组(134例子宫内膜癌患者和95名健康女性),采用基体辅助激光解吸电离飞行时间质谱(matrix-associated laser disso-ciation/ionization time of flight mass spectrmtry,MALDI-TOF-MS)结合纳米磁珠技术测定其血清多肽谱,Biomarker Wizard软件筛选差异蛋白峰,采用SPSS 12.0中ROC曲线分析差异峰建立诊断模型,进一步评价模型对诊断子宫内膜癌的敏感度和特异性,并分析差异蛋白峰在各期子宫内膜癌及子宫良性病变患者中的分布情况。结果血清多肽谱检测结果显示,测试组质荷比为1 500~30 000m/z,共测得82个蛋白峰,其中有12个差异峰差异有统计学意义,P0.001。在12个差异峰中,取ROC曲线面积≥0.8的2 768、3 402和6 441m/z 3个差异峰作为子宫内膜癌的诊断模型,其敏感度分别为78.4%、75.4%和66.8%,特异性分别为92.6%、89.5%和86.2%;三者联合的敏感度和特异性为88.1%和75.8%。联合诊断模型鉴别子宫内膜癌和子宫良性病变的敏感度为94.9%。结论血清多肽谱检测筛查发现的差异峰2 768、3 402和6 441m/z能较正确的区分子宫内膜癌患者、健康人和子宫良性病变患者,有助于子宫内膜癌的早期筛查。
[Abstract]:Objective to study the changes of serum polypeptide spectrum in patients with endometrial carcinoma and to explore the differential proteins associated with early diagnosis of endometrial carcinoma. Methods 134 cases of endometrial carcinoma, 103 cases of uterine body benign lesion and 95 healthy female volunteers were selected from Zhejiang Provincial Cancer Hospital from January to 31, 2008- 05-01-2011-05-31. The subjects included 134 patients with endometrial carcinoma, 103 patients with benign uterine body disease and 95 healthy women volunteers. The subjects were divided into two groups: 45 patients with stage I endometrial carcinoma and 45 healthy women, and 134 patients with endometrial carcinoma and 95 healthy women in the test group. Matrix-associated laser disso-ciation/ionization was used in matrix assisted laser desorption ionization time of flight mass spectrometry. Time of flight mass spectrum MALDI-TOF-MS combined with nano-magnetic bead technique was used to determine the serum polypeptide spectrum and Biomarker Wizard software to screen the differential protein peaks. The differential peak of SPSS 12.0 was used to establish a diagnostic model to evaluate the sensitivity and specificity of the model in the diagnosis of endometrial carcinoma, and to analyze the distribution of differential protein peak in patients with endometrial carcinoma and benign lesions. Results the results of serum polypeptide spectrum showed that the mass charge ratio of the tested group was 1 500 ~ 30 000 m / z, and 82 protein peaks were detected, of which 12 of them showed significant difference (P 0.001). Among the 12 differential peaks, the sensitivity and specificity of the three peaks with ROC curve area 鈮，

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