盆腔器官脱垂患者阴道壁和子宫骶、主韧带组织中神经纤维特点和VIP、NPY蛋白的表达

发布时间：2018-05-09 06:37

本文选题：盆腔器官脱垂 + 血管活性肠肽　；参考：《郑州大学学报(医学版)》2015年02期

【摘要】：目的:研究盆腔器官脱垂(POP)患者阴道壁及子宫骶、主韧带的组织结构特点和神经纤维损伤情况,以及血管活性肠肽(VIP)、神经肽Y(NPY)的表达与POP疾病的关系。方法:选择POP轻度患者7例、重度患者8例,另取20例非POP患者作对照。选取患者阴道前壁和子宫骶、主韧带组织,采用HE染色进行病理组织学观察,免疫组化染色检测神经纤维标记PGP9.5蛋白,血管周围VIP、NPY蛋白的表达。结果:POP轻、重度组患者产次均高于对照组(F=12.196,P0.001)。POP患者阴道壁及子宫骶、主韧带组织结构薄弱,神经纤维萎缩。3组神经纤维密度比较差异无统计学意义(P0.05)。3组阴道壁及子宫骶、主韧带组织中血管周围VIP、NPY蛋白的表达差异有统计学意义(F=17.312、19.825、18.312、16.327、20.721、22.625,P0.05),POP组高于对照组,POP重度组高于轻度组。结论:产次可能是POP发生的高危因素。POP患者阴道壁,子宫骶、主韧带组织中神经纤维萎缩。VIP、NPY在POP的发生、发展中起重要作用。
[Abstract]:Objective: to study the structural characteristics and nerve fiber damage of vaginal wall, uterus sacral and main ligaments in patients with pelvic organ prolapse (POP), and the relationship between the expression of vasoactive intestinal peptide (VIPP) and neuropeptide (YNPY) and POP disease. Methods: 7 cases of mild POP, 8 cases of severe POP and 20 cases of non-POP were selected as control group. The anterior wall of vagina and the sacral and main ligament tissues of the patients were selected to observe the histopathology by HE staining. The expression of PGP9.5 protein labeled by nerve fibers and the expression of VIPP PGP9.5 protein around the blood vessels were detected by immunohistochemical staining. Results the vaginal wall and uterus sacrum were higher in the severe group than that in the control group (P < 0.01). The tissue structure of the main ligament was weak. There was no significant difference in the density of nerve fiber in the group of atrophy of nerve fiber (P 0.05). There was significant difference in the expression of VIPP NPY protein in the perivascular tissue of the main ligament. There was a significant difference in the expression of VIP-NPY protein between the two groups. The expression of VIP-NPY protein in the perivascular tissue was significantly higher in the control group than in the control group. The expression of VIP-NPY protein in the perivascular tissue was significantly higher in the control group than in the mild group. Conclusion: the birth time may be the high risk factor of POP. The nerve fiber atrophy in vagina wall, uterus sacral and main ligament tissue in patients with POP may play an important role in the occurrence and development of POP.
【作者单位】：河南大学淮河医院妇产科;广西医科大学第一附属医院妇产科;
【基金】：广西壮族自治区科技厅基金支持项目桂科攻10124001A-46
【分类号】：R711.2

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