母鼠营养不良导致子代在生命早期出现糖脂代谢紊乱及其机制探讨

发布时间：2018-05-11 01:40

本文选题：母体营养 + 糖脂代谢　；参考：《遗传》2015年01期

【摘要】：为了探讨母鼠孕期和哺乳期营养不良对子代生命早期糖脂代谢的影响及其机制,文章对孕期和哺乳期母鼠分别喂养高脂饮食、低蛋白饮食和正常饮食,观察其子鼠断乳时(3周龄)糖脂代谢指标,并采用荧光定量PCR方法检测子鼠肝组织氧化物酶增殖物激活受体γ(PPARγ)基因的表达情况。结果表明:子鼠在3周龄时,与正常饮食组相比,低蛋白饮食组子鼠出生体重(7.36±0.91 vs 8.94±1.39,P0.0001)较低,体长较短(12.27±0.53 vs13.44±0.36,P0.0001);高脂饮食组子鼠体重(9.53±0.68 vs 7.36±0.91,P0.0001)和体长(13.22±0.35 vs 12.27±0.53,P0.0001)均高于低蛋白饮食组;另外,高脂饮食组子鼠腹腔糖耐量实验30 min和60 min血糖明显高于正常饮食组(P0.001),且高脂饮食组30 min血糖水平也明显高于低蛋白饮食组(P0.001),高脂饮食组子鼠糖耐量曲线下面积明显大于正常饮食组(P0.001)。另外,与正常饮食组相比,高脂饮食组子鼠空腹胆固醇水平明显升高(1.64±0.21 vs 1.18±0.16,P0.01),低蛋白饮食组空腹胆固醇水平明显下降(0.96±0.09 vs 1.18±0.16,P0.05)。荧光定量PCR结果显示,在低蛋白饮食组和高脂饮食组,其子鼠肝组织PPARγ基因表达量均明显高于正常饮食组(P0.05)。结果显示,母鼠妊娠期和哺乳期高脂饮食与低蛋白饮食均可以诱导子鼠在发育早期出现糖脂代谢紊乱,PPARγ基因可能在其中参与了重要的调控作用。
[Abstract]:In order to investigate the effect of maternal malnutrition during pregnancy and lactation on the metabolism of glucose and lipid in the early life of offspring, the high fat diet, low protein diet and normal diet were fed to pregnant and lactating mothers, respectively. The indexes of glucose and lipid metabolism were observed at the age of 3 weeks during weaning, and the expression of PPAR- 纬 gene in liver tissue was detected by fluorescence quantitative PCR. The results showed that compared with the normal diet group, the birth weight of the offspring in the low-protein diet group was 7.36 卤0.91 vs 8.94 卤1.39 P0.0001) and the body length was 12.27 卤0.53 vs13.44 卤0.36 P0.0001) in the low protein diet group, 9.53 卤0.68 vs 7.36 卤0.91g P0.0001 in the high-fat diet group, and 13.22 卤0.35 vs 12.27 卤0.53P0.0001 in the low protein diet group, and the body length was 13.22 卤0.35 vs 12.27 卤0.53P0.0001 in the high-fat diet group. The blood glucose levels in the high fat diet group were significantly higher than those in the normal diet group for 30 min and 60 min, and the blood glucose level in the high fat diet group at 30 min was significantly higher than that in the low protein diet group. The area under the glucose tolerance curve in the high fat diet group was significantly higher than that in the low protein diet group. It was significantly higher than that of normal diet group (P 0.001). In addition, compared with the normal diet group, the fasting cholesterol level in the high-fat diet group was significantly higher than that in the high-fat diet group (1.64 卤0.21 vs 1.18 卤0.16 P0.01g), and the fasting cholesterol level in the low-protein diet group was significantly lower than that in the low-protein diet group (0.96 卤0.09 vs 1.18 卤0.16 P0.05). The results of fluorescence quantitative PCR showed that the expression of PPAR 纬 gene was significantly higher in the liver tissues of the rats in the low protein diet group and the high fat diet group than that in the normal diet group (P 0.05). The results showed that both high fat diet and low protein diet during pregnancy and lactation could induce the disorder of glucose and lipid metabolism in the early stage of development. PPAR 纬 gene might play an important role in the regulation.
【作者单位】：中国医学科学院北京协和医学院北京协和医院内分泌科卫生部内分泌重点实验室;
【基金】：国家自然科学基金项目(编号:81170736)资助
【分类号】：R714.25

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