精液优选对精子DNA碎片的影响及其对体外受精-胚胎移植妊娠结局的影响

发布时间：2018-05-11 02:38

本文选题：精子染色质扩散法 + 精子DNA碎片　；参考：《实用医学杂志》2017年17期

【摘要】：目的评估密度梯度离心联合上游法改善精子DNA碎片(sperm DNA fragmentation)的效率及其对体外受精-胚胎移植(IVF-ET)妊娠结局的影响。方法收集120份处理前后的精液,用精子染色质扩散法检测精子DNA碎片,评估其对100个新鲜移植患者妊娠结局的影响。结果优选后,男性不育组精子DNA碎片率(SDFI)由22.75(14.44,30.25)降至11.5(5.60,22.79),对照组则由20.86(15.00,26.81)降至7.5(3.63,15.44);妊娠组精子DNA碎片明显低于非妊娠组,ROC曲线下面积是0.667;高水平组(DFI≥20%)的妊娠率和出生率低于低水平组(DFI20%),差异有统计学意义。结论密度梯度离心联合上游法可减少精子DNA碎片;优选后DNA碎片对IVF-ET妊娠结局具有预测价值,一旦超过阈值,虽可正常受精,但妊娠率和出生率下降。
[Abstract]:Objective to evaluate the efficiency of density gradient centrifugation combined with upstream method in improving sperm DNA fragment sperm DNA fragmentation and its effect on in vitro fertilization and embryo transfer (IVF-ETT) pregnancy outcome. Methods 120 semen samples were collected before and after treatment. Sperm DNA fragments were detected by sperm chromatin diffusion method and their effects on pregnancy outcome of 100 fresh transplant patients were evaluated. Results after the election, The rate of sperm DNA fragments in male infertility group decreased from 22.75 to 11.55.605.60,22.79g, and that in control group decreased from 20.86v 15.0026.81) to 7.53.63c15.440.The area under the curve of sperm DNA fragment in pregnancy group was significantly lower than that in non-pregnancy group (0.667); the pregnancy rate and birth rate in high level group were lower than those in non-pregnancy group. The difference was statistically significant in group D I 20. Conclusion density gradient centrifugation combined with upstream method can reduce sperm DNA fragments, and DNA fragments after optimal selection can predict the pregnancy outcome of IVF-ET. Once above the threshold value, the pregnancy rate and birth rate are decreased, although normal fertilization can be achieved.
【作者单位】：广东省生殖医学重点实验室广东省产科重大疾病重点实验室广东省普通高校生殖与遗传重点实验室广州医科大学第三附属医院生殖医学中心;
【分类号】：R714.8

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