种植窗期uNK对血管生成的影响及子宫动脉血流与胚胎种植的相关性研究

发布时间：2018-05-12 05:09

本文选题：体外受精-胚胎移植 + 种植窗期　；参考：《中国免疫学杂志》2015年10期

【摘要】：目的:探讨体外受精-胚胎移植患者种植窗期子宫内膜自然杀伤细胞(u NK)对子宫内膜微血管的影响及子宫动脉搏动指数与胚胎种植的关系。方法:回顾性分析本院2009年1月至2013年7月实施体外受精-胚胎移植(IVF-ET)的37例患者的临床资料,根据是否妊娠成功及至少2次行IVF-ET失败分为成功组(26例)和失败组(11例),比较两组研究对象种植窗期的子宫内膜u NK细胞标记物CD56+、子宫动脉搏动指数、血管内皮标记物F8因子、ɑ平滑肌肌动蛋白(ɑSMA)及重链肌球蛋白(SMM)的表达差异及其相互关系。结果:成功组的基础FSH 3.52±0.68(m U/ml)、基础窦卵泡9.44±2.53(个)与失败组比较差异均不具有统计学意义(P0.05)。成功组的u NK细胞27.18±5.94(%)、MVD 6.79±1.74(%)、ɑSMA 33.72±4.19(%)、SMM 25.19±5.83(%)表达率均显著高于IVF-ET助孕失败组(P0.05)。种植窗期37例患者的子宫内膜u NK细胞比例与MVD数目、ɑSMA及SMM着色细胞数比例均呈显著的正相关性[(r=0.472,P=0.0000.005)、(r=0.426,P=0.0000.005)、(r=0.512,P=0.0000.05)]。成功组种植窗期的子宫动脉PI=0.94±0.28,失败组的PI=0.87±0.29,两组比较差异不具有统计学意义(t=0.688,P=0.4930.05)。结论:种植窗期的子宫内膜组织中自然杀伤细胞与子宫内膜血管生成具有显著的相关性,可能与成功妊娠具有一定的关系;子宫动脉PI与妊娠结局是否成功关系不明显。
[Abstract]:Aim: to investigate the effect of natural killer cells (NK) on endometrial microvessel and the relationship between uterine artery pulsatility index and embryo implantation in patients with in vitro fertilization and embryo transfer. Methods: the clinical data of 37 patients who underwent IVF-ETT from January 2009 to July 2013 were retrospectively analyzed. According to the success of pregnancy and the failure of IVF-ET at least 2 times, the success group (n = 26) and the failure group (n = 11) were divided into two groups. The CD56, uterine artery pulsatile index, a NK cell marker in implantation window, was compared between the two groups. The expression of vascular endothelial markers F8 factor, smooth muscle actin (SMAs) and heavy chain myosin (SMMM) were different and their correlation was observed. Results: there was no significant difference in basic FSH (3.52 卤0.68mU / ml) and basic antral follicle (9.44 卤2.53) between the successful group and the failed group (P 0.05). In the successful group, the expression rate of SMA was significantly higher than that in the failed IVF-ET group (27.18 卤5.94) and SMA 33.72 卤4.19 卤25.19 卤5.83, respectively. There was a significant positive correlation between the percentage of endometrial NK cells and the number of MVD, SMA and SMM staining cells in the endometrium of 37 patients at the implantation window stage. The uterine artery PI=0.94 卤0.28 in the successful group and PI=0.87 卤0.29 in the failed group were not significantly different between the two groups. Conclusion: there is a significant correlation between natural killer cells and endometrial angiogenesis in implantation window phase, which may be related to successful pregnancy, but there is no significant relationship between uterine artery Pi and pregnancy outcome.
【作者单位】：郑州大学第一附属医院生殖中心;
【分类号】：R714.8

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