PCOS与抑郁对雌鼠下丘脑单胺类神经递质和血清黄体生成素、睾酮分泌的影响

发布时间：2018-05-12 05:26

本文选题：多囊卵巢综合征 + 抑郁　；参考：《福建医科大学学报》2017年03期

【摘要】：目的研究多囊卵巢综合征(PCOS)及抑郁2种因素对雌性大鼠下丘脑中单胺类神经递质[多巴胺(DA)、去甲肾上腺素(NE)、5-羟色胺(5-HT)、促性腺素释放素(GnRH)]及血清中黄体生成素(LH)、睾酮(T)分泌的影响,分析抑郁与PCOS之间的相互关系。方法将40只雌性6周龄清洁级SD大鼠分为:空白组、抑郁组及PCOS组及PCOS合并抑郁组。采用放射免疫法测定大鼠血清T及LH水平;ELISA法检测大鼠下丘脑中GnRH水平;高效液相色谱-荧光检测流速梯度法检测大鼠下丘脑中NE,DA及5-HT水平。结果 4组大鼠血清LH及T水平、下丘脑GnRH水平比较,差别具有统计学意义(P0.05),且呈递增趋势。4组大鼠下丘脑的DA,NE及5-HT水平比较,差别具有统计学意义(P0.05),NE呈递增趋势,DA及5-HT呈递减趋势。GnRH变化趋势与DA(P0.05,r=-0.874)、5-HT(P0.05,r=-0.821)变化趋势呈负相关,而与NE变化趋势呈正相关(P0.05,r=0.809)。结论 PCOS及抑郁2种因素均会使机体中枢DA,NE及5-HT分泌异常,影响下丘脑GnRH、血清LH及T的分泌,影响雌性大鼠的神经内分泌系统,引起机体卵巢组织学、临床表征及心理等发生变化;抑郁可能是PCOS发生及产生远期并发症的重要诱因,而PCOS又可导致抑郁的发生及加剧其发展。
[Abstract]:Objective to study the effects of polycystic ovary syndrome (PCOS) and depression on monoamine neurotransmitters in hypothalamus of female rats and serum luteinizing hormone (LHH). The effect of ketone on T secretion, To analyze the relationship between depression and PCOS. Methods 40 female 6 week old clean SD rats were divided into three groups: blank group, depression group, PCOS group and PCOS combined depression group. Serum T and LH levels in rats were measured by radioimmunoassay and GnRH levels in hypothalamus were detected by Elisa, and NEDA and 5-HT levels in hypothalamus of rats were detected by high performance liquid chromatography-fluorescence detection flow rate gradient method. Results the levels of serum LH, T and GnRH in hypothalamus were significantly higher in the four groups than in the control group (P 0.05), and the levels of DANE and 5-HT in hypothalamus of the 4 groups showed an increasing trend. The difference was statistically significant. The trend of DA and 5-HT decreased gradually. GnRH was negatively correlated with the trend of DAP0.05r-0.874 and 5-HTP0.05r-0.821, but positively correlated with the trend of NE change (P0.05r0.809). Conclusion both PCOS and depression can make the secretion of DANNE and 5-HT in the body center abnormal, affect the secretion of GnRH, LH and T in hypothalamus, affect the neuroendocrine system of female rats, and cause the histology of ovary. Depression may be an important inducement for the occurrence and long-term complications of PCOS, and PCOS may lead to the occurrence and exacerbation of depression.
【作者单位】：福建医科大学附属协和医院妇产科;福建医科大学附属协和医院神经外科;
【分类号】：R711.75;R749.4

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