性激素结合球蛋白、胰岛素信号转导蛋白和葡萄糖转运蛋白在妊娠期糖尿病胎盘组织中的表达及相关性分析

发布时间：2018-05-14 01:12

本文选题：妊娠期糖尿病 + 性激素结合蛋白　；参考：《中国医科大学学报》2017年02期

【摘要】：目的研究妊娠期糖尿病(GDM)孕妇胎盘组织中性激素结合球蛋白(SHBG)、胰岛素信号转导蛋白及葡萄糖转运蛋白表达,探讨其在GDM发病过程中的作用。方法收集足月妊娠且非肥胖(BMI25 kg/m2)GDM孕妇(GDM组)和同期糖代谢正常(正常组)孕妇胎盘组织各10例,应用Western blotting、实时PCR方法检测2组胎盘组织中SHBG和胰岛素信号转导蛋白(IRS-1、ISR-2、PI3K p85α)和葡萄糖转运蛋白(GLUT-1、GLUT-3、GLUT-4)的表达,各指标进行直线回归依存关系分析。结果与正常组比较,GDM组胎盘组织中SHBG m RNA、蛋白表达降低(P0.05);IRS-1蛋白、IRS-2 m RNA表达降低(P0.05);PI3K p85α和GLUT-1蛋白及其m RNA表达无统计学差异(P0.05);GLUT-3蛋白,GLUT-4 m RNA、蛋白表达降低(P0.05)。直线回归分析结果显示SHBG m RNA与IRS-2 m RNA、PI3K p85αm RNA、GLUT-4 m RNA的表达呈正相关(均P0.05);IRS-2 m RNA和GLUT-4 m RNA的表达呈正相关(P0.01);IRS-1 m RNA和PI3K p85αm RNA、GLUT-3 m RNA的表达呈负相关(P0.05);IRS-2m RNA和GLUT-1 m RNA的表达正相关(P0.01)。结论 GDM孕妇胎盘中胰岛素信号转导和葡萄糖转运蛋白存在异常,SHBG可能参与胰岛素信号通路的调节,GDM时胎盘滋养细胞合成和分泌SHBG减少,引起胰岛素信号转导通路相关蛋白表达降低,从而导致胰岛素抵抗及GDM的发生。
[Abstract]:Objective to study the expression of neutral hormone binding globulin (SHBG), insulin signal transduction protein and glucose transporter protein in pregnant women with gestational diabetes mellitus (GDM), and to explore its role in the pathogenesis of GDM. Methods the placenta of pregnant women of full-term pregnancy and non obese (BMI25 kg/m2) GDM pregnant women (group GDM) and normal group of glucose metabolism (normal group) were collected. The expression of SHBG and insulin signal transduction proteins (IRS-1, ISR-2, PI3K p85 a) and glucose transporters (GLUT-1, GLUT-3, GLUT-4) in the 2 groups of placental tissues were detected by Western blotting. The results were compared with the normal group. The results were compared with the normal group. The expression of white expression decreased (P0.05), and the expression of IRS-1 protein and IRS-2 m RNA decreased (P0.05); PI3K p85 A and GLUT-1 protein and m RNA expressed no statistical difference (P0.05). There is a positive correlation between the expression of IRS-2 m RNA and GLUT-4 m RNA (P0.01), IRS-1 m RNA and PI3K p85. At GDM, the synthesis of placental trophoblast cells and the secretion of SHBG decrease, resulting in a decrease in the expression of protein related proteins in the insulin signal transduction pathway, which leads to insulin resistance and the occurrence of GDM.

【作者单位】：中国医科大学附属盛京医院妇产科;
【基金】：国家自然科学基金(81170591) 沈阳市科技计划(F11-262-9-46)
【分类号】：R714.256

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