脐血流异常对选择性宫内生长受限代谢组学影响的初步探讨

发布时间：2018-05-15 12:50

本文选题：单绒毛膜双胎 + 选择性宫内生长受限　；参考：《现代妇产科进展》2017年08期

【摘要】：目的:探讨合并脐血流异常对选择性宫内生长受限(s IUGR)脐血血浆代谢组学的影响。方法:纳入6对合并脐血流异常及3对脐血流正常(对照组)的s IUGR双胎,利用气相色谱-飞行时间质谱技术(GC-TOF/MS)对双胎脐血进行代谢物检测,通过主成分分析(PCA)和正交偏最小二乘判别分析(OPLS-DA)对质谱数据进行多元变量模式识别分析,根据模型的变量投影重要性、学生t检验筛选可能的标记物,并进行代谢通路分析。结果:合并脐血流异常s IUGR筛选出17种有统计学意义的差异代谢物。其中谷氨酸、草酸、环亮氨酸、N-(2-羟乙基)亚氨基二乙酸及L-犬尿氨酸在s IUGR合并脐血流异常组增加,天冬氨酸、N-乙酰-L-天冬氨酸、乳酸、L-苏糖、酪氨酸、甘氨酸、富马酸、β-甘露糖基甘油酸、β-丙氨酸、亚油酸、棕榈酸和马来酰胺酸则降低。结论:GC-TOF/MS方法证实合并脐血流异常的s IUGR代谢组学发生改变,主要体现在脂质、碳水化合物、氨基酸、能量、核酸、辅助因子和维生素代谢等方面。
[Abstract]:Objective: to investigate the effect of umbilical blood flow abnormality on plasma metabolism in selective intrauterine growth restriction (IUGR). Methods: six pairs of s IUGR twins with abnormal umbilical blood flow and 3 pairs of normal umbilical blood flow (control group) were included. The metabolites of umbilical cord blood were detected by gas chromatography-time of flight mass spectrometry (GC-TOF / MS). The multivariate pattern recognition analysis of mass spectrum data was carried out by means of principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). According to the importance of variable projection of the model, the student t test was used to screen the possible markers and the metabolic pathway was analyzed. Results: 17 different metabolites with abnormal umbilical blood flow were screened by s IUGR. Glutamic acid, oxalic acid, cycloleucine N-ketoxyethyl) iminodiacetic acid and L-canine uric acid were increased in IUGR with abnormal umbilical blood flow, aspartic acid was N-acetyl-L-aspartic acid, L-threonose lactate, tyrosine, glycine, Fumaric acid, 尾 -mannose glyceric acid, 尾 -alanine, linoleic acid, palmitic acid and maleic acid decreased. Conclusion the changes of s IUGR metabolism in patients with abnormal umbilical blood flow were confirmed by the% GC-TOF / MS method, mainly in the metabolism of lipids, carbohydrates, amino acids, energy, nucleic acids, cofactors and vitamins.
【作者单位】：北京大学第三医院妇产科;
【基金】：“生殖健康及重大出生缺陷防控研究”重点专项(项目编号2016YFC1000400,课题编号2016YFC1000408)
【分类号】：R714.5

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