纳秒电脉冲致人卵巢癌细胞DNA损伤的时间效应

发布时间：2018-05-17 10:44

本文选题：纳秒电脉冲 + 卵巢癌　；参考：《生物医学工程学杂志》2015年05期

【摘要】：本研究旨在探讨纳秒电脉冲(nsEP)对人卵巢癌细胞铂敏感株A2780及铂耐药株C30的DNA损伤效应。将A2780和C30分别作用于场强为6kV/cm,脉宽为24ns的脉冲电场处理60s。假处理组予假脉冲处理。nsEP处理后0h、4h、8h、12h和24h采用CCK-8法检测细胞生存率;采用碱性单细胞凝胶电泳实验(彗星实验)检测细胞DNA损伤,测定彗星尾长(TL)、尾矩(TM)和Olive尾矩(OTM)的75th百分位数。结果显示随着时间增加,A2780和C30细胞的生存率和彗星形成率均先降低后增加,8h时最低(P0.05);C30细胞的生存率高于A2780细胞(P0.05),彗星形成率低于A2780细胞(P0.05);C30细胞的TL、TM和OTM均低于A2780细胞(P0.05);细胞死亡率和彗星形成率呈正相关(r=0.997,P0.05;r=0.998,P0.05)。结果表明,nsEP对人卵巢癌铂敏感细胞株和铂耐药细胞株的DNA损伤效应存在差异;nsEP致细胞死亡与DNA损伤相关。
[Abstract]:The purpose of this study was to investigate the DNA damage effects of nanosecond electrical pulse nsEPs on platinum sensitive human ovarian cancer cell line A2780 and platinum resistant cell line C30. A2780 and C30 were treated with a pulsed electric field with a field strength of 6 kV / cm and pulse width of 24ns for 60 s, respectively. In the sham treatment group, the cell survival rate was detected by CCK-8 assay at 0 h, 8 h and 24 h after treatment with pseudopulse. The cell DNA damage was detected by alkaline single cell gel electrophoresis (comet assay). The 75th percentiles of Olive and Olive were measured. The results showed that the survival rate and comet formation rate of A2780 and C30 cells decreased first and then increased at 8h. The survival rate of C30 cells was higher than that of A2780 cells, and the comet formation rate was lower than that of A2780 cells. The TLTM and OTM of A2780 cells were lower than that of A2780 cells. There was a positive correlation between cell mortality and comet formation rate. The results showed that DNA damage induced by nsEP was associated with DNA damage in platinum-sensitive and platinum-resistant human ovarian cancer cell lines.
【作者单位】：重庆医科大学附属第二医院妇产科;重庆市食品药品检验所;
【基金】：国家自然科学基金资助项目(31470822) 高等学校博士学科点专项科研基金资助项目(20135503130002,20125503110004)
【分类号】：R737.31

【共引文献】