玻璃化冷冻胚胎与新鲜胚胎移植对子代小鼠生长发育的影响

发布时间：2018-05-18 05:41

本文选题：体外受精 + 新鲜胚胎　；参考：《医学研究生学报》2015年06期

【摘要】：目的辅助生殖技术会引起子代缺陷,文中通过比较经体外受精(invitro fertilization,IVF)的新鲜胚胎与玻璃化冷冻胚胎移植后所产生的子代小鼠生长状况,以探讨胚胎冷冻对IVF后代生长发育的影响。方法实验共分为3组,对照组(12只):小鼠自然交配所产生子代;鲜胚移植组(9只):收集IVF后的小鼠囊胚,直接将囊胚进行移植;冻胚移植组(9只):收集IVF后的小鼠囊胚,进行玻璃化冷冻胚胎,复苏后移植到假孕母鼠子宫内,分析比较3组子代小鼠体重的变化,性成熟期体脂。结果鲜胚移植组与冻胚移植组胚胎在高倍显微镜下形态无明显差异。冻胚移植组0~12周子代小鼠体重高于对照组(P0.01),鲜胚移植组子代小鼠0周、2~6周体重高于对照组(P0.05);冻胚移植组性成熟期脂肪含量[(9.32±4.34)g]高于鲜胚组[(5.24±2.56)g]和对照组[(2.80±0.48)g],差异有统计学意义(P0.05)。冻胚移植组肌肉含量[(33.77±4.22)g]高于对照组[(27.50±1.79)g],差异有统计学意义(P0.01)。结论鲜胚移植技术对子代的生长状况干扰小于玻璃化冷冻技术。
[Abstract]:Objective to study the effect of embryo freezing on the growth of IVF progeny by comparing the growth status of fresh embryos and vitrified frozen embryos after transplantation of fresh embryos and vitrified frozen embryos. Methods the experiment was divided into three groups: control group (n = 12): offspring produced by natural mating of mice; fresh embryo transplantation group (n = 9): collection of mouse blastocyst after IVF and direct transplantation of blastocyst; frozen embryo transplantation group (n = 9): collection of mouse blastocyst after IVF, Vitrified frozen embryos were transplanted into the womb of female pseudopregnant mice after resuscitation. The changes of body weight and body fat during sexual maturity were analyzed and compared among the 3 groups. Results there was no significant difference in morphology between fresh embryo transfer group and frozen embryo transfer group under high power microscope. The body weight of chilled embryo transplantation group was higher than that of control group at 12 weeks, the body weight of fresh embryo transplantation group was higher than that of control group at 0 weeks, and the body weight of fresh embryo transplantation group was higher than that of control group at 2 weeks, and the fat content of frozen embryo transplantation group was higher than that of fresh embryo group [5.24 卤2.56g] and control group (2.80 卤0.48g), the difference was statistically significant (P 0.05). The muscle content of frozen embryo transplantation group [33.77 卤4.22 g] was higher than that of control group [27.50 卤1.79 g], and the difference was statistically significant (P 0.01). Conclusion the influence of fresh embryo transplantation on the growth of offspring is less than that of vitrification.
【作者单位】：江苏大学临床医学院;南京军区南京总医院生殖医学中心;
【基金】：国家自然科学基金(31371520)
【分类号】：R714.8

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