子痫前期孕妇体内S100A12水平检测及其血清对滋养细胞增殖、凋亡和RAGE表达的影响

发布时间：2018-05-18 15:11

本文选题：子痫前期 + SA　；参考：《郑州大学学报(医学版)》2015年03期

【摘要】：目的:检测子痫前期孕妇外周血和胎盘组织中S100A12蛋白的表达,观察其血清对滋养细胞的影响,探讨S100A12与子痫前期的关系。方法:选择轻度子痫前期孕妇30例,重度子痫前期孕妇30例,同期入院分娩的正常孕妇30例(正常对照组),采用ELISA法检测孕妇外周血中S100A12水平,免疫组化法检测胎盘组织中S100A12蛋白的表达水平。用各组孕妇血清体外培养滋养细胞48 h,另设无血清培养的空白对照组,应用MTT法和流式细胞仪检测细胞增殖及凋亡情况,Western blot法检测细胞中晚期糖基化终末产物受体RAGE蛋白的表达。结果:轻、重度子痫前期组孕妇外周血及胎盘组织中S100A12水平均高于正常对照组(P0.05),且重度子痫前期组高于轻度子痫前期组(P0.05)。轻、重度子痫前期组滋养细胞早期凋亡率、晚期凋亡率、总凋亡率及RAGE蛋白的表达均明显高于正常孕妇及空白对照组(P0.05),增殖率则降低(P0.05);重度子痫前期组较轻度子痫前期组变化更显著(P0.05)。结论:S100A12高表达可能是子痫前期的重要发病机制之一。
[Abstract]:Aim: to detect the expression of S100A12 protein in peripheral blood and placenta of pregnant women with preeclampsia, to observe the effect of serum on trophoblast, and to explore the relationship between S100A12 and preeclampsia. Methods: 30 cases of mild preeclampsia, 30 cases of severe preeclampsia and 30 cases of normal pregnant women were selected. The levels of S100A12 in peripheral blood of pregnant women were detected by ELISA method. The expression of S100A12 protein in placenta was detected by immunohistochemical method. The trophoblastic cells were cultured in vitro for 48 hours with the serum of pregnant women in each group, and a blank control group was set up without serum culture. MTT assay and flow cytometry were used to detect cell proliferation and apoptosis. Western blot assay was used to detect the expression of advanced glycation end product receptor (RAGE). Results: the levels of S100A12 in the peripheral blood and placenta in the mild and severe preeclampsia group were higher than those in the normal control group (P 0.05), and the levels in the severe preeclampsia group were higher than that in the mild preeclampsia group (P 0.05). In the mild and severe preeclampsia group, the rate of early apoptosis and late apoptosis of trophoblast, The total apoptotic rate and the expression of RAGE protein were significantly higher than those of normal pregnant women and blank control group (P 0.05), while the proliferative rate of severe preeclampsia group was significantly higher than that of mild preeclampsia group (P 0.05), while the proliferation rate of severe preeclampsia group was significantly higher than that of mild preeclampsia group. Conclusion the high expression of S100 A 12 may be one of the important pathogenesis of preeclampsia.
【作者单位】：郑州大学第一附属医院妇产科;
【分类号】：R714.244

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