肝X受体在卵巢癌组织中的表达及T0901317在卵巢癌细胞系SKOV3中的作用

发布时间：2018-05-20 05:11

本文选题：卵巢肿瘤 + 肝X受体　；参考：《山东大学学报(医学版)》2017年01期

【摘要】：目的检测肝X受体蛋白在卵巢癌组织及正常卵巢组织中的表达,探讨肝X受体激动剂T0901317对卵巢癌细胞系SKOV3细胞活力及凋亡的影响。方法收集2015年8月至2016年8月行初次手术治疗的51例卵巢癌患者的组织标本,另选取32例因子宫肌瘤等良性病变切除的正常卵巢组织作对照。采用免疫组织化学方法检测肝X受体蛋白在卵巢癌组织及正常卵巢组织中的表达;CCK8检测T0901317对卵巢癌细胞系SKOV3增殖的影响;流式细胞术检测T0901317对卵巢癌细胞系SKOV3凋亡的影响。结果肝X受体蛋白在正常卵巢组织较卵巢癌组织表达量高,两组比较差异有统计学意义(P0.001),T0901317抑制卵巢癌细胞系SKOV3增殖具有明显的剂量依赖性和时间依赖性(P0.05),T0901317促进SKOV3凋亡具有明显的剂量依赖性(P0.05)。结论肝X受体表达下调可能与卵巢癌的发生发展有关,激活肝X受体可能会成为卵巢癌潜在的治疗靶点。
[Abstract]:Objective to detect the expression of liver X receptor protein in ovarian cancer and normal ovarian tissues, and to investigate the effects of T0901317, a hepatic X receptor agonist, on the viability and apoptosis of ovarian cancer cell line SKOV3. Methods from August 2015 to August 2016, the tissue specimens of 51 patients with ovarian cancer underwent initial surgical treatment were collected and 32 normal ovarian tissues were selected as control group, which were excised from benign lesions such as uterine leiomyoma. The expression of liver X receptor protein in ovarian cancer and normal ovarian tissues was detected by immunohistochemical method. The effect of T0901317 on the proliferation of ovarian cancer cell line SKOV3 and the effect of T0901317 on apoptosis of ovarian cancer cell line SKOV3 were detected by flow cytometry. Results the expression of X receptor protein in normal ovary was higher than that in ovarian cancer. There was a significant difference between the two groups in the inhibition of SKOV3 proliferation by P0.001T0901317 in a dose-dependent and time-dependent manner. P0.05T0901317 promoted SKOV3 apoptosis in a dose-dependent manner. Conclusion the down-regulation of liver X receptor expression may be related to the occurrence and development of ovarian cancer, and activation of liver X receptor may be a potential therapeutic target for ovarian cancer.
【作者单位】：山东大学齐鲁医院妇产科;
【基金】：山东省重点研发计划(2015GGE27352)
【分类号】：R737.31

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