妊娠期肝内胆汁淤积症胎盘OATP1A2和OATP1B3的表达及其多态性研究

发布时间：2018-05-21 10:55

本文选题：妊娠期肝内胆汁淤积症 + 胎盘　；参考：《现代妇产科进展》2017年04期

【摘要】：目的:探讨胎盘OATP1A2和OATP1B3的表达水平及OATP1B3基因多态性与妊娠期肝内胆汁淤积症(ICP)的关系。方法:收集42例ICP胎盘和42例正常妊娠胎盘标本及母体静脉血,RT-q PCR和Western blot法分析OATP1A2和OATP1B3的mRNA及蛋白水平,测序法分析OATP1B3 c.334TG和c.699GA多态性位点基因型。结果:ICP胎盘中OATP1A2和OATP1B3的mRNA及蛋白表达水平均显著低于正常妊娠组(P0.05)。ICP组和正常妊娠组中,OATP1B3 c.334CT多态性纯合突变频率分别为50.0%和47.6%(P0.05),c.699GA多态性纯合突变频率分别为54.8%和45.2%(P0.05);两组的两种多态性的突变等位基因频率比较,差异均无统计学意义(70.2%vs 70.2%和73.8%vs 67.9%,P0.05)。ICP组OATP1B3不同基因型对其mRNA及蛋白的表达无显著影响(P0.05)。结论:ICP胎盘中OATP1A2和OATP1B3表达下调可能与ICP高胆汁酸的发生有关,而OATP1B3 c.334TG和c.699GA多态性不影响OATP1B3表达。
[Abstract]:Objective: to investigate the expression of OATP1A2 and OATP1B3 in placenta and the relationship between OATP1B3 gene polymorphism and intrahepatic cholestasis of pregnancy. Methods: the mRNA and protein levels of OATP1A2 and OATP1B3 were analyzed by RT-q PCR and Western blot in 42 ICP placentas and 42 normal pregnancy placentas, and OATP1B3 c.334TG and c.699GA polymorphism genotypes were analyzed by sequencing. Results the mRNA and protein expression levels of OATP1A2 and OATP1B3 in the placenta were significantly lower than those in the normal pregnancy group (P0.05A). The frequencies of homozygous mutation of OATP-1B3 c.334CT polymorphism were 50.0% and 47.6%, respectively. The frequencies of homozygous mutation were 54.8% and 45.2%, respectively. The frequency of mutation alleles in the two polymorphic groups was compared. There was no significant difference between the two groups (70.2% vs 70.2% and 73.8%vs 67.9%). The different genotypes of OATP1B3 in ICP group had no significant effect on the expression of mRNA and protein. Conclusion the down-regulation of OATP1A2 and OATP1B3 expression in the placenta of ICP may be related to the occurrence of hyperbile acid in ICP, while the polymorphism of OATP1B3 c.334TG and c.699GA does not affect the expression of OATP1B3.
【作者单位】：郑州大学附属郑州中心医院妇产科;郑州大学临床医学系;郑州大学基础医学院药理学系;
【基金】：河南省科技厅基础与前沿技术研究项目(No:152300410052)
【分类号】：R714.255

【相似文献】