高危型人乳头瘤病毒感染的子宫颈非典型鳞状细胞中p16基因的缺失

发布时间：2018-05-22 16:43

本文选题：子宫颈肿瘤 + 上皮内瘤变　；参考：《临床与实验病理学杂志》2015年06期

【摘要】：目的观察p16基因在高危型人乳头瘤病毒(human papillomavirus,HPV)感染的子宫颈非典型鳞状细胞(atypical squamous cells of undermined significance,ASCUS)中的缺失,探讨其作为子宫颈病变筛查的临床意义。方法收集27例高危型HPV-DNA检测为阳性、液基薄层细胞学(thinprep cytologic test,TCT)诊断为ASCUS的脱落细胞标本。27例患者均在1个月内进行阴道镜检查并行组织学活检,根据组织学结果分组:对照组(包括炎症和HPV感染)7例;子宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)组20例,其中CIN1 3例、CIN2 13例、CIN3 4例。制作细胞蜡块并切片,采用FISH技术检测ASCUS中p16基因缺失情况。结果p16基因在对照组和CIN组中的缺失率分别为14.3%和85.0%,二者差异有统计学意义(P0.01)。结论采用FISH技术检测ASCUS细胞中p16基因缺失有助于从伴有ASCUS患者中筛选出子宫颈CIN患者。
[Abstract]:Objective to observe the deletion of p16 gene in atypical squamous cell of cervix (HPV) infected by human papillomavirus (HPV), and to explore its clinical significance as a screening method for cervical lesions. Methods 27 exfoliated cell samples were collected from 27 cases of high risk HPV-DNA positive and diagnosed as ASCUS by thin layer cytology of liquid base. All the 27 patients underwent colposcopy and histological biopsy within one month. According to histological results, 20 cases were divided into control group (including 7 cases of inflammation and HPV infection), cervical intraepithelial neoplasia cin group (20 cases), including 3 cases of CIN1 and 13 cases of CIN3 cases. Cell wax blocks were made and sections were made. FISH technique was used to detect the deletion of p16 gene in ASCUS. Results the deletion rates of p16 gene in control group and CIN group were 14.3% and 85.0%, respectively. Conclusion Detection of p16 gene deletion in ASCUS cells by FISH is helpful to screen out cervical CIN patients with ASCUS.
【作者单位】：广东省深圳市龙岗区人民医院病理科;广东省深圳市龙岗区人民医院妇产科;
【基金】：深圳市卫生与计划生育委员会项目(201402123) 深圳市龙岗区科技局资助项目(YS2013044)
【分类号】：R737.33

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