SPARC、E-cadherin、EZH2在子宫内膜样腺癌中的蛋白表达及临床意义

发布时间：2018-05-22 17:52

本文选题：子宫内膜样腺癌 + SPARC　；参考：《兰州大学》2014年硕士论文

【摘要】：目的：检测SPARC、E-cadherin及EZH2在增殖期子宫内膜(proliferative phase endometrium, PPE)、子宫内膜单纯性增生(simple endometrial hyperplasia, SEH)、子宫内膜复杂性增生(complex endometrial hyperplasia,CEH)、子宫内膜非典型增生(atypical endometrial hyperplasia,AEH)及子宫内膜样腺癌(endometrioid adenocarcinoma, EA)组织中的蛋白表达,探讨三种蛋白表达与EA临床病理因素之间的关系及其在EA发生、发展中的作用。方法：采用免疫组化S-P法检测PPE、SEH、CEH、AEH及EA组织中SPARC、 E-cadherin、EZH2的表达情况。结果： 1、SPARC、E-cadherin在EA组织中的阳性表达率分别是29.23%和27.69%,明显低于PPE、SEH、CEH、AEH组织中二者的表达,差异具有统计学意义(P0.01)；而EZH2在EA组织中的阳性表达率为73.85%,明显高于PPE、SEH、 CEH、AEH的组织中的阳性表达,差异具有统计学意义(P0.01)。 2、在EA组织中,SPARC、E-cadherin的阳性表达与肿瘤的组织病理分级、肌层浸润深度及淋巴结转移有关,差异具有统计学意义(P0.05)；而EZH2的阳性表达与肿瘤的组织病理分级、肌层浸润深度有关,差异具有统计学意义(P0.05),而与淋巴结转移无关(P0.05)。 3、在EA组织中,SPARC和E-cadherin的表达呈正相关(rs=0.226,P0.05)；SPARC和EZH2的表达呈负相关(rs=-0.201,P0.05)；E-cadherin和EZH2的表达呈负相关(rs=-0.214,P0.01)。结论： 1、从PPE、SEH、CEH、AEH到EA组织,SPARC、E-cadherin的蛋白表达呈递减趋势,而EZH2的表达呈递增趋势,提示三者不同表达可能与EA的癌变发生有关。 2、SPARC、E-cadherin、EZH2表达与EA的病理分级、肌层浸润密切相关；SPARC、E-cadherin表达还与淋巴结转移密切相关。提示三者不同表达可能在EA的发展、浸润和转移等过程中起重要作用。 3、SPARC、E-cadherin及EZH2在EA中的表达呈现相关性,提示三种蛋白在EA的发生、发展中可能存在相互作用,联合检测SPARC、E-cadherin及EZH2将有助于EA的临床诊断、治疗及疗效评价。
[Abstract]:Objective: To detect the expression of SPARCn E-cadherin and EZH2 in proliferative phase endometrium, PPEI, simple endometrial hyperplasia, SEH, endometrial hyperplasia CEH, atypical endometrial hyperplasia (AEH) and endometrioid adenocarcinoma (EAA) in proliferative endometrium and endometrial carcinoma, the expression of SPARCU E-cadherin and EZH2 in proliferative phase endometrium (PPE), simple endometrial hyperplasia (EZH2), endometrial complex hyperplasia (endometrial), atypical endometrial hyperplasia (AEH) and endometrial adenocarcinoma (EAA) were detected. To explore the relationship between the expression of three proteins and the clinicopathological factors of EA and its role in the development and development of EA. Methods: Immunohistochemical S-P method was used to detect the expression of SPARC, E-cadherin EZH _ 2 in the tissues of PPE-SEHH-CEHH and EA. Results: (1) the positive expression rates of E-cadherin in EA were 29.23% and 27.69%, respectively, which were significantly lower than those in PPESEHCEH and AEH, and the difference was statistically significant (P0.01), while the positive rate of EZH2 in EA was 73.85, which was significantly higher than that in PPESEHand CEHAEH. The difference was statistically significant (P 0.01). 2. The positive expression of E-cadherin was related to the histological grade, the depth of myometrial invasion and lymph node metastasis in EA, but the positive expression of EZH2 was related to the histopathological grade and the depth of myometrial invasion. The difference was statistically significant (P 0.05), but not related to lymph node metastasis (P 0.05). 3. There was a positive correlation between the expression of SPARC and E-cadherin in EA tissues. There was a negative correlation between the expression of SPARC and EZH2 in EA tissues. There was a negative correlation between the expression of E-cadherin and EZH2. Conclusion: 1. The protein expression of SPARCU E-cadherin decreased from PPE-SEH CEH to EA, while the expression of EZH2 increased, suggesting that the different expression of AEH might be related to the carcinogenesis of EA. 2the expression of E-cadherin was closely related to the pathological grade of EA, the expression of E-cadherin in muscularis and the expression of E-cadherin in SPARC+ E-cadherin was also closely related to lymph node metastasis. These results suggest that the different expressions may play an important role in the development, infiltration and metastasis of EA. 3The expressions of E-cadherin and EZH2 in EA were correlated, suggesting that there might be interaction between the three proteins in the development and occurrence of EA. The combined detection of E-cadherin and EZH2 will be helpful to the clinical diagnosis, treatment and evaluation of curative effect of EA.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

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