eIF4A在卵巢上皮性癌中表达及其与预后的关系

发布时间：2018-05-24 06:37

本文选题：真核细胞起始因子A + 卵巢上皮性癌　；参考：《现代妇产科进展》2015年10期

【摘要】：目的:检测真核细胞起始因子4A(e IF4A)在不同卵巢组织中的表达,探讨其临床意义及其与预后的关系。方法:利用荧光实时定量PCR及Western blot法检测42例卵巢上皮性癌(EOC)组织、15例卵巢交界性上皮性肿瘤组织、15例卵巢良性上皮性肿瘤组织和20例正常卵巢组织中e IF4A mRNA及蛋白的表达情况,同时采用免疫组化SP法检测123例EOC石蜡切片中e IF4A蛋白表达情况,并评估影响患者总生存率的高危因素。结果:EOC中e IF4AmRNA及蛋白表达显著高于交界性、良性及正常卵巢上皮组织(P0.05);交界性上皮肿瘤组织明显高于良性及正常卵巢上皮组织(均P0.05);卵巢良性上皮性组织略高于正常卵巢组织,但差异无统计学意义(P0.05)。EOC中e IF4A表达与FIGO手术病理分期、组织学分级、淋巴结转移及腹水情况有关(P均0.05),而与年龄及组织学类型无关(P0.05)。Kaplan-Meier生存曲线显示,e IF4A蛋白高表达者的总生存率明显低于低表达者(Log-Rank检验,P0.05)。COX比例风险模型分析发现,临床病理分期、组织学分级及术后有无残余瘤灶是EOC患者的独立预后因素(P0.05)。结论:e IF4A在EOC的发生进展中可能起重要作用,有可能成为判断预后的指标之一。
[Abstract]:Aim: to detect the expression of eukaryotic initiation factor (4A(e IF4A) in different ovarian tissues and to explore its clinical significance and its relationship with prognosis. Methods: 15 cases of ovarian borderline epithelial tumors and 15 cases of benign epithelial tumors of ovary and 20 cases of normal ovarian tissues were detected for e IF4A mRNA and e IF4A mRNA by fluorescence real time quantitative PCR and Western blot method in 42 cases of ovarian epithelial carcinoma and 15 cases of ovarian borderline epithelial tumors. The expression of protein, Immunohistochemical SP method was used to detect the expression of e IF4A protein in 123 cases of EOC paraffin sections and to evaluate the high risk factors affecting the overall survival rate. Results the expression of e IF4AmRNA and protein in EOC was significantly higher than that in borderline tissues, and the expression of e IF4AmRNA and protein in benign and normal ovarian epithelial tissues was significantly higher than that in benign and normal ovarian epithelial tissues (P0.05), and in borderline epithelial tumor tissues was significantly higher than that in benign and normal ovarian epithelial tissues (P0.05), and in benign ovarian epithelial tissues was slightly higher than that in normal ovarian tissues. But there was no significant difference in the expression of e IF4A in P0.05. EOC and the pathological stage and histological grade of FIGO. Lymph node metastasis and ascites were all related to P 0.05, but the overall survival rate was significantly lower in patients with high expression of IF4A than that in patients with low expression of P0.05U. Kaplan-Meier survival curve. Histological grade and postoperative residual tumor were independent prognostic factors of EOC patients (P 0.05). Conclusion IF4A may play an important role in the pathogenesis and progression of EOC and may be one of the prognostic markers.
【作者单位】：青岛大学附属医院妇科;
【分类号】：R737.31

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