白细胞介素25促进早孕期母-胎界面滋养细胞的增殖

发布时间：2018-05-24 06:48

本文选题：IL- + 滋养细胞　；参考：《现代免疫学》2017年05期

【摘要】：探索人早孕绒毛组织和滋养细胞中白细胞介素25(interleukin 25,IL-25)及其受体的表达,以及IL-25对滋养细胞的促增殖作用。免疫组织化学法检测IL-25在正常早孕期绒毛组织的表达;流式细胞术检测IL-25及其受体在正常妊娠和自然流产绒毛组织的滋养细胞中的表达;CCK-8实验分析重组IL-25(rhIL-25)及其中和性抗体对人绒毛膜滋养细胞株HTR-8/SVneo细胞活力的影响。结果显示IL-25在正常绒毛组织滋养细胞中呈阳性表达,自然流产患者绒毛滋养细胞的IL-25及其受体的表达均低于正常妊娠(P0.001);重组IL-25处理后,HTR-8/SVneo细胞活力以时间依赖和剂量依赖方式显著增强(P0.001或P0.000 1);相反,IL-25中和性抗体明显抑制其细胞活力(P0.05或P0.01或P0.001)。结果表明人早孕母-胎界面滋养细胞表达IL-25,以自分泌方式促进自身增殖,IL-25异常低表达可能参与反复自然流产的发病机制。
[Abstract]:To explore the expression of interleukin 25(interleukin 25 (IL 25) and its receptor in human chorionic villi and trophoblast, and the effect of IL-25 on the proliferation of trophoblast. Immunohistochemical method was used to detect the expression of IL-25 in the villi of normal early pregnancy. The expression of IL-25 and its receptors in trophoblastic cells of human chorionic villi of normal pregnancy and spontaneous abortion was detected by flow cytometry. The effects of recombinant IL-25 rhIL-25) and its neutralizing antibody on the activity of human choriotrophoblastic HTR-8/SVneo cells were analyzed by CCK-8 assay. The results showed that IL-25 was positively expressed in trophoblast of normal villi. The expression of IL-25 and its receptors in chorionic trophoblastic cells in spontaneous abortion patients was lower than that in normal pregnant women (P 0.001), and the activity of HTR-8 / SVneo cells was significantly increased in a time-and dose-dependent manner after treatment with recombinant IL-25. On the contrary, the neutralizing antibodies against IL-25 were obvious. The cell activity was inhibited by P0.05 or P0.01 or P0.001. The results showed that the expression of IL-25 in the trophoblastic cells at the maternal and fetal interface of human early pregnancy may be involved in the pathogenesis of recurrent spontaneous abortion, and the abnormal low expression of IL-25 may be involved in the autocrine promotion of self-proliferation.
【作者单位】：复旦大学上海医学院附属妇产科医院暨妇产科研究所生殖免疫教研室;上海交通大学附属上海市第一人民医院妇产科;
【基金】：国家自然科学基金(81571509) 上海市自然科学基金(16ZR1427900) 上海市人才发展基金(2016014)
【分类号】：R714.21

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