Ang-1、Ang-2、TSP-1与复发性流产的相关性研究

发布时间：2018-05-24 12:30

本文选题：复发性流产 + 血管生成素-1　；参考：《山西医科大学》2014年硕士论文

【摘要】：目的通过观察复发性流产(RSA)患者绒毛、蜕膜组织中血管生成素-1(Ang-1)、血管生成素-2(Ang-2)、血小板反应蛋白-1(TSP-1)的表达情况，探讨Ang-1、Ang-2、TSP-1与RSA的关系，为临床治疗提供理论依据。方法选择2013年5月--2013年11月在山西大医院妇产科门诊行早期人工流产的复发性流产患者30例为研究组，选择同期自愿行人工流产、既往分娩过足月正常胎儿的正常早孕妇女30例为对照组，在人工流产的同时留取绒毛及蜕膜组织，经免疫组化SP法检测Ang-1、Ang-2及TSP-1在两组绒毛及蜕膜组织中的表达水平，并对有关数据进行统计分析。结果 1. Ang-1、Ang-2、TSP-1在各组每例的绒毛及蜕膜中均有表达。研究组绒毛组织中Ang-1、Ang-2的表达高于对照组，TSP-1的表达低于对照组；蜕膜组织中Ang-1、Ang-2、TSP-1的表达均低于对照组，且差异均有统计学意义(P＜0.05)。研究组绒毛Ang-1/Ang-2比值高于对照组，且差异有统计学意义(P＜0.05)；研究组蜕膜中Ang-1/Ang-2比值高于对照组，但无统计学意义(P0.05)。 2.研究组中，绒毛Ang-1、Ang-2及TSP-1表达均高于蜕膜，Ang-1/Ang-2比值低于蜕膜，且差异有统计学意义(P＜0.05)。 3.对照组中，绒毛中Ang-1表达显著低于蜕膜，TSP-1表达高于蜕膜，且差异有统计学意义(P＜0.05)，Ang-2在绒毛、蜕膜组织中表达无统计学差异(P0.05)，绒毛Ang-1/Ang-2比值低于蜕膜，且有统计学差异(P＜0.05)。结论 1.Ang-1、Ang-2、TSP-1在反复自然流产患者绒毛及蜕膜组织的异常表达可能是造成复发性流产的原因。 2. Ang-1、Ang-2在蜕膜的低表达可能是导致其在绒毛中的高表达及Ang-1/Ang-2平衡失调的原因，，两者共同作用导致局部血管生成障碍及流产的发生。 3. RSA患者绒毛组织中Ang-1过度表达对血管生成缺陷的影响可能更大。 4. TSP-1在绒毛及蜕膜中的低表达可能也是导致血管生成紊乱及RSA的原因。
[Abstract]:Purpose By observing the expression of Angiopoietin-1 (Ang-1), Ang-1 (Ang-1), Angiopoietin-2 (Ang-2) and platelet-reactive protein (-1) in the villi and decidua of patients with recurrent abortion, we investigated the relationship between Ang-1 and RSA, and provided a theoretical basis for clinical treatment. Method From May 2013 to November 2013, 30 patients with recurrent abortion who underwent early abortion in outpatient department of gynecology and obstetrics of Shanxi Great Hospital were selected as study group, and voluntary abortion was performed in the same period. 30 normal early pregnant women who had delivered term normal fetuses were used as control group. Villi and decidua tissues were taken simultaneously after induced abortion. The expression of Ang-1Ang-2 and TSP-1 in villi and decidua of the two groups were detected by immunohistochemical SP method. And carries on the statistical analysis to the related data. Result 1. Ang-1 and Ang-2 TSP-1 were expressed in villi and decidua of each group. The expression of Ang-1Ang-2 in chorionic villi in the study group was higher than that in the control group (P < 0.05), and that in decidua tissue was lower than that in the control group (P < 0.05). The ratio of Ang-1/Ang-2 in the study group was higher than that in the control group (P < 0.05) and the ratio of Ang-1/Ang-2 in decidua in the study group was higher than that in the control group (P < 0.05). 2. In the study group, the expression of Ang-1Ang-2 and TSP-1 in chorionic villi was higher than that in decidua Ang-1 / Ang-2 ratio, and the difference was statistically significant (P < 0.05). 3. In the control group, the expression of Ang-1 in chorionic villi was significantly lower than that in decidua, and the difference was statistically significant (P < 0.05). There was no significant difference in the expression of Ang-1/Ang-2 in chorionic villi. The Ang-1/Ang-2 ratio of villi was lower than that of decidua (P < 0.05). Conclusion 1. The abnormal expression of TSP-1 in villi and decidua of patients with recurrent spontaneous abortion may be the cause of recurrent abortion. 2. The low expression of Ang-1Ang-2 in decidua may be the cause of high expression of Ang-1Ang-2 in villi and imbalance of Ang-1/Ang-2 balance. 3. Overexpression of Ang-1 in chorionic villi of RSA patients may have a greater effect on angiogenesis defects. 4. The low expression of TSP-1 in chorionic villi and decidua may also be the cause of angiogenesis disorder and RSA.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.21

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