HPV16E7,E5序列多态性与新疆地区维，汉妇女宫颈癌及癌前病变的相关性研究

发布时间：2018-05-26 17:19

本文选题：HPV16E7和E5 + 序列多态性　；参考：《石河子大学》2014年硕士论文

【摘要】：目的：本研究检测HPV16E7，E5序列变异在新疆地区维吾尔族，汉族妇女宫颈癌及癌前病变中的发生情况，，探讨HPV16E7，E5基因变异率在新疆地区维吾尔族，汉族妇女不同宫颈病变中的相关性。方法：对HPV16DNA阳性的119例宫颈炎（维54例，汉65例）、70例CINI（维30例，汉40例）、91例CINII-CINIII(维45例，汉46例)和105例宫颈癌（维60例，汉45例），对其E7和E5PCR扩增进行测序后对比，分析突变情况。结果：除了HPV16E7和E5的原型序列以外，HPV16E7最常见的变异为A647G和T846C，主要以A647G突变为主，A647G变异株频率在不同宫颈病变中为宫颈炎（维22.2%，汉24.6%）、CINI（维60.0%，汉55.0%）、CINII-CINIII（维57.8%，汉60.9%）、宫颈癌（维55.3%，汉66.7%）；HPV16E5最常见的变异为A4042G和A3978C,主要以A4042G突变为主，A4042G变异株频率在不同宫颈病变中为宫颈炎（维18.5%，汉21.5%）、CINI（维53.3%，汉45.0%）、CINII-CINIII（维53.3%，汉56.5%）、宫颈癌（维55.0%，汉68.9%）；HPV16E7A647G变异株和HPV16E5A4042G变异株在新疆地区维吾尔族，汉族妇女各个宫颈病变阶段中的总体分布差异有统计学意义（P0.05），比较维吾尔族，汉族妇女宫颈癌及癌前病变中的HPV16E7A647G变异株和HPV16E5A4042G变异株的分布差异无统计学意义（P0.05）。结论：HPV16E7A647G变异株和HPV16E5A4042G变异株是新疆地区维吾尔族，汉族妇女宫颈癌及癌前病变中流行的变异株，此变异株在维吾尔族，汉族妇女各个宫颈病变阶段中的总体分布有差异性，即此变异株与宫颈病变的程度有相关性；在维吾尔族，汉族妇女宫颈癌及癌前病变中的分布比较无差异性，即此变异株在新疆地区宫颈病变中无民族差异性。
[Abstract]:Objective: to investigate the prevalence of HPV16E7E5 gene mutation in Uygur and Han women in Xinjiang, and to explore the correlation of HPV16E7E5 gene mutation in different cervical lesions of Uygur and Han women in Xinjiang. Methods: 119 cases of HPV16DNA positive cervicitis (54 cases of cervicitis, 65 cases of Han and 70 cases of CINI (30 cases), 40 cases of CINII-CINIII (45 cases) and 105 cases of cervical carcinoma (60 cases, 45 cases of cervical carcinoma) were analyzed by sequencing. Analyze the mutation. Results: in addition to the prototype sequences of HPV16E7 and E5, the most common variants of HPV16E7 were A647G and T846C.The frequency of A647G mutation was mainly A647G mutation. The frequency of A647G variant was cervicitis in different cervical lesions. 55.3The most common variation of HPV16E5 was A4042G and A3978C.The main frequency of A4042G mutation was cervicitis in different cervical lesions. The Uyghurs in Xinjiang, The distribution of HPV16E7A647G variant and HPV16E5A4042G variant in cervical cancer and precancerous lesion of Han women were not significantly different between Uygur and Han women (P 0.05). Conclusion the HPV16E5A4042G variant strain and the 1% HPV16E7A647G variant strain are the most prevalent variant strains in the cervical cancer and precancerous lesions of Uygur and Han women in Xinjiang. There are differences in the overall distribution of this variant in all stages of cervical lesions in Uygur and Han women. There was no difference in the distribution of cervical cancer and precancerous lesions among Uygur and Han women, that is to say, there was no ethnic difference in cervical lesions in Xinjiang.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

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