TWEAK通过外泌体途径介导巨噬细胞抑制上皮性卵巢癌细胞转移的研究

发布时间：2018-05-29 14:17

本文选题：TWEAK + 卵巢癌　；参考：《现代妇产科进展》2015年07期

【摘要】：目的:探讨TWEAK能否通过外泌体途径介导巨噬细胞对上皮性卵巢癌细胞转移的调控。方法:佛波酯诱导人单核细胞系THP-1为贴壁的巨噬细胞,TWEAK刺激巨噬细胞24h后抽提外泌体,透射电镜观察其形态;分别将PBS、巨噬细胞分泌的外泌体、TWEAK刺激后巨噬细胞分泌的外泌体与上皮性卵巢癌细胞系SKOV3、HO-8910pm共培养,48h后通过Transwell实验检测其迁移、侵袭能力。结果:透射电镜可见巨噬细胞分泌的外泌体呈圆形,直径30~100nm。Transwell实验显示,与空白对照组相比,巨噬细胞分泌的外泌体可使上皮性卵巢癌细胞的迁移和侵袭能力增高;而TWEAK刺激巨噬细胞后,其分泌的外泌体则可降低其迁移和侵袭能力。结论:TWEAK可通过外泌体途径逆转巨噬细胞对上皮性卵巢癌细胞的促转移作用。
[Abstract]:Aim: to investigate whether TWEAK mediates macrophages' regulation of epithelial ovarian cancer cell metastasis through exocrine pathway. Methods: phorbol ester induced human monocytes THP-1 as adherent macrophages to stimulate macrophages for 24 hours, then extract exocrine bodies, and observe their morphology by transmission electron microscope (TEM). PBSs, macrophages secreted by TWEAK and epithelial ovarian cancer cell line SKOV3HO-8910pm were co-cultured for 48 hours. The migration and invasion ability were detected by Transwell assay. Results: the exocrine bodies secreted by macrophages were round by transmission electron microscope. The diameter 30~100nm.Transwell test showed that the exocrine secreted by macrophages could increase the migration and invasion ability of epithelial ovarian cancer cells compared with the blank control group. However, after TWEAK stimulated macrophages, the exocrine secreted by macrophages decreased their migration and invasion ability. Conclusion: TWEAK can reverse the role of macrophages in promoting metastasis of epithelial ovarian cancer cells through exocrine pathway.
【作者单位】：上海交通大学医学院附属仁济医院妇产科上海市妇科肿瘤重点实验室;
【分类号】：R737.31

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