用基因芯片技术筛选人子宫内膜癌细胞株中紫杉醇耐药相关基因

发布时间：2018-05-29 17:16

本文选题：基因芯片技术 + 子宫肿瘤　；参考：《山东医药》2015年19期

【摘要】：目的应用基因芯片技术筛选人子宫内膜癌细胞株中获得性紫杉醇(TAX)耐药相关基因。方法采用大剂量间歇诱导法,用TAX反复冲击诱导培养人子宫内膜癌Ishikawa细胞株,建成Ishikawa/TAX耐药株。应用Affymetrix Human Genome U133 Plus2.0表达谱芯片检测Ishikawa/TAX与Ishikawa细胞株基因。通过Gene Ontology(GO)聚类分析对差异表达的基因予以分类。采用qRT-PCR对部分差异表达的基因进行验证。结果与Ishikawa细胞相比,Ishikawa/TAX细胞中有110条基因表达有显著性差异(ratio比值3),包括87个上调基因、23个下调基因,GO分类涉及细胞信号传导和调控、细胞黏附分子、细胞代谢及转录调控等;其中上调最明显的基因为S100A12,其次有CYP1B1、FUBP1、CEACAM6,下调基因有TNFAIP3、CD44、DKK1等。qRT-PCR检测S100A12、CYP1B1在耐药细胞中高表达,TNFAIP3、CD44在耐药细胞中低表达,与基因芯片结果一致。结论采用基因芯片技术筛选出的人子宫内膜癌获得性TAX耐药相关基因的上调基因有S100A12、CYP1B1、FUBP1、CEACAM6,下调基因有TNFAIP3、CD44、DKK1。
[Abstract]:Objective to screen acquired paclitaxel (TAX) resistance related genes in human endometrial cancer cell lines by gene chip technique. Methods Human endometrial carcinoma Ishikawa cell line was induced by high dose intermittent induction and repeated shock of TAX. Ishikawa/TAX resistant strain was established. Ishikawa/TAX and Ishikawa cell lines were detected by Affymetrix Human Genome U133 Plus2.0 expression microarray. The differentially expressed genes were classified by Gene ontology cluster analysis. Some differentially expressed genes were verified by qRT-PCR. Results the expression of 110 genes in Ishikawa / tat cells was significantly different from that in Ishikawa cells, including 87 up-regulated genes and 23 down-regulated genes involved in cell signal transduction and regulation, cell adhesion molecules, cell metabolism and transcriptional regulation. The most obvious up-regulated gene was S100A12, followed by CYP1B1FUBP1CEACAM6, and down-regulated gene was TNFAIP3pCD4DKK1. QRT-PCR was used to detect the high expression of S100A12CYP1B1 in resistant cells, which was consistent with the results of gene chip. Conclusion the up-regulated genes associated with acquired TAX resistance in human endometrial carcinoma screened by gene chip technique are S100A12CYP CYP1B1 FUBP1 CEACAM6, and down-regulated genes are TNFAIP3CD4DK1.
【作者单位】：首都医科大学附属北京世纪坛医院;
【分类号】：R737.33

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