CD44v6、整合素αvβ3、EGFR及E-钙粘素在A549和SK-OV-3细胞系的表达差异与远处转移关系的研究

发布时间：2018-05-31 15:54

本文选题：A549 + SK-OV-3　；参考：《川北医学院》2014年硕士论文

【摘要】：目的：利用流式细胞仪检测肺癌A549细胞系和卵巢癌SK-OV-3细胞系中CD44v6、整合素αvβ3、EGFR及E-钙粘素的表达水平差异，进一步探讨这些差异与肺癌和卵巢癌远处转移的关系。方法：培养肺癌A549细胞和卵巢癌SK-OV-3细胞生长至对数期，消化并收集细胞悬液，调整细胞浓度至流式细胞仪专用抗体所要求的浓度。利用流式细胞仪分组检测两种细胞系CD44v6、整合素αvβ3、EGFR及E-钙粘素的表达水平，每组6个样本。数据采用SPSS19.0软件（中文版）进行统计学分析，计量资料组间差异采用两独立样本t检验，检验水准为P＜0.05。结果：CD44v6在肺癌A549细胞系和卵巢癌SK-OV-3细胞系中表达的阳性率分别为（93.48±2.97）%和（82.73±4.28）%；整合素αvβ3在肺癌A549细胞株和卵巢癌SK-OV-3细胞系中表达的阳性率分别为（95.42±2.26）%和（83.60±1.26）%；EGFR在肺癌A549细胞系和卵巢癌SK-OV-3细胞系中表达的阳性率分别为（94.30±3.43）%和（88.52±0.88）%。CD44v6、整合素αvβ3、EGFR这三种表面分子在肺癌A549细胞系中的表达均高于卵巢癌SK-OV-3细胞系，有统计学意义（P＜0.01）。E-钙粘素在肺癌A549细胞系和卵巢癌SK-OV-3细胞系中表达的阳性率分别为（94.52±1.59）%和（86.96±3.07）%，，有统计学意义（P＜0.05）。结论：促转移分子CD44v6、整合素αvβ3、EGFR在肺癌A549细胞系的表达高于卵巢癌SK-OV-3细胞系，抑制转移分子E-钙粘素在肺癌A549细胞系的表达也高于卵巢癌SK-OV-3细胞系，综合考虑，这可能是肺癌比卵巢癌更容易发生远处转移特别是脑转移的部分分子机制。
[Abstract]:Objective: to detect the difference in expression of CD44v6, integrin alpha v beta 3, EGFR and E- cadherin in lung cancer A549 cell lines and ovarian cancer SK-OV-3 cell lines by flow cytometry, and to further explore the relationship between these differences and distant metastasis of lung cancer and ovarian cancer.
Methods: the growth of lung cancer A549 cells and ovarian cancer SK-OV-3 cells grew to the logarithmic phase, digested and collected the cell suspension and adjusted the concentration required by the cell concentration to the specific antibody of the flow cytometry. The expression level of two cell lines, integrin alpha v beta 3, EGFR and E- cadherin was detected by flow cytometry, with 6 samples in each group. SPSS19.0 software (Chinese version) was used for statistical analysis, and two independent samples t test was used to measure the difference between the data groups. The test level was P < 0.05..
Results: the positive rates of CD44v6 in lung cancer A549 cell line and ovarian cancer SK-OV-3 cell line were (93.48 + 2.97)% and (82.73 + 4.28)%, respectively, and the positive rates of integrin alpha v beta 3 in A549 cell line and SK-OV-3 cell line of ovarian cancer were (95.42 + 2.26)% and (83.60 + 1.26)% respectively, EGFR in A549 cell line and ovarian cancer SK- in lung cancer SK- The positive rates of expression in OV-3 cell lines were (94.30 + 3.43)% and (88.52 + 0.88)%.CD44v6, integrin alpha v beta 3, EGFR and three surface molecules in A549 cell lines of lung cancer were higher than those of ovarian cancer SK-OV-3 cell lines. There were statistical significance (P < 0.01) the positive expression of.E- cadherin in lung cancer A549 cell line and ovarian cancer SK-OV-3 cell line. The sex ratio was (94.52 + 1.59)% and (86.96 + 3.07)% respectively, and the difference was statistically significant (P < 0.05).
Conclusion: the expression of CD44v6, integrin alpha v beta 3, EGFR in the A549 cell line of lung cancer is higher than that of the ovarian cancer SK-OV-3 cell line. The expression of E- cadherin in the A549 cell line of lung cancer is higher than that of the ovarian cancer SK-OV-3 cell line, which may be more likely to occur in the lung cancer than in the ovarian cancer, especially in the distant metastasis, especially in the brain metastasis. A part of the molecular mechanism.
【学位授予单位】：川北医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R734.2;R737.31

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