子痫前期患者胎盘及脐带组织IDO、MIF表达变化及意义

发布时间：2018-06-01 05:15

本文选题：子痫前期 + 吲哚胺　；参考：《山东医药》2015年12期

【摘要】：目的观察子痫前期(PE)患者胎盘及脐带组织吲哚胺2,3-双加氧酶(IDO)、巨噬细胞移动抑制因子(MIF)的表达变化,并探讨其意义。方法 PE患者66例,其中轻度32例(轻度组)、重度34例(重度组),同期健康晚孕妇女42例作为对照组。分别采用RT-PCR法和Western blot法检测胎盘、脐带组织中IDO、MIF mRNA和蛋白,用彩色超声多普勒诊断仪检测各组产前胎儿的脐动脉血流阻力指数(RI),记录新生儿体质量。结果重度组、轻度组、对照组胎盘、脐带组织IDO mRNA及蛋白相对表达均逐渐升高,MIF mRNA及蛋白相对表达、RI均逐渐降低;组间两两比较,P均0.01。重度组新生儿体质量均低于轻度组和对照组,P均0.01。PE患者中,胎盘、脐带组织IDO、MIF mRNA相对表达均呈负相关(r分别为-0.468、-0.455),P均0.01;胎盘、脐带组织IDO mRNA表达水平与RI均呈负相关(r分别为-0.317、-0.526),与新生儿体质量均呈正相关(r分别为0.398、0.461),P均0.01;胎盘、脐带组织MIF mRNA表达水平与RI均呈正相关(r分别为0.393、0.502),与新生儿体质量均呈负相关(r分别为-0.302、-0.431),P均0.01。结论 PE患者胎盘及脐带组织中IDO表达降低、MIF表达升高,与胎儿RI升高和新生儿体质量降低有关,可能参与PE的发病。
[Abstract]:Objective to observe the expression of indoleamine 2o 3-dioxygenase (IDO) and macrophage migration inhibitor (MIF) in placenta and umbilical cord of patients with preeclampsia (PEE) and to explore its significance. Methods 66 patients with PE were included in this study, including 32 mild cases (mild group), 34 severe cases (severe group) and 42 healthy women with late pregnancy as control group. RT-PCR method and Western blot method were used to detect IDOOMIF mRNA and protein in placenta and umbilical cord respectively. The umbilical artery blood flow resistance index (RI) was measured by color Doppler echocardiography in each group, and the body weight of newborns was recorded. Results the relative expression of IDO mRNA and protein in placenta and umbilical cord tissues of severe group, mild group and control group were all increased gradually, and the relative expression of mRNA and protein RI were decreased gradually, and there was a significant difference between the two groups (P < 0.01). The relative expression of MIF mRNA in placenta and umbilical cord was negatively correlated with the weight of neonates in severe group and control group (P < 0. 01, P = 0. 468, P = 0. 455P, P = 0. 01, respectively), placenta, umbilical cord and placenta. There was a negative correlation between the expression of IDO mRNA and RI in umbilical cord tissues, and the positive correlation between the expression of IDO mRNA and the body weight of the newborn was -0.317- 0.526, respectively, and the positive correlation between the expression of IDO mRNA and the body weight of the newborn was 0.3980.461P, 0.01respectively, placenta, and placenta. There was a positive correlation between the expression of MIF mRNA and RI in umbilical cord tissues, and a negative correlation between the expression of MIF mRNA and the body weight of newborns (P < 0.01). Conclusion the decrease of IDO expression in placenta and umbilical cord of PE patients may be related to the increase of fetal RI and the decrease of neonatal body mass, which may be involved in the pathogenesis of PE.
【作者单位】：沧州医学高等专科学校;沧州市传染病医院;沧州市人民医院;
【基金】：河北省高等学校科学技术研究青年基金资助项目(QN20132002)
【分类号】：R714.244

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