转化生长因子-β对人卵巢癌细胞系SK-OV-3活性氧簇表达的影响及其机制

发布时间：2018-06-03 18:26

  本文选题：转化生长因子-β + 人卵巢癌细胞系　； 参考：《山东医药》2017年31期

【摘要】：目的观察转化生长因子-β(TGF-β)对人卵巢癌细胞系SK-OV-3活性氧簇(ROS)表达的影响,并探讨其可能机制。方法取对数生长期SK-OV-3细胞,随机分为两组,观察组用2 mL培养液+2μL无血清DMEM配制的5μg/mL TGF-β培养(最终浓度为5 ng/mL),对照组直接加2 mL培养液。流式细胞仪检测细胞内总的活性氧簇(ROS)产生及线粒体来源的ROS,实时荧光定量PCR检测细胞上皮细胞-间充质转化(EMT)变化各指标、线粒体呼吸链复合体Ⅲ组分mRNA。结果观察组及对照组总ROS产生量分别为3 159±42.92、1 062±24.41,两组比较,P0.05。观察组及对照组细胞内线粒体来源ROS产生量分别为1 254±29.04、1 039±17.32,两组比较,P0.05。观察组E-cadherin、N-cadherin、Vimentin、ZEB1分别是对照组的0.200 0、6.739 8、4.487 9、1.639 5倍,P均0.05。观察组CYC1、TTC19、UQCRC1、UQCRC2、UQCR10、UQCRB、UQCRFS1、UQCRH、UQCRQ mRNA表达水平较对照组改变倍数分别为0.89、1.04、1.09、0.87、1.17、1.18、0.92、1.09、1.07倍,P均0.05。观察组CYC1、TTC19、UQCRC1、UQCRC2、UQCRFS1蛋白水平较对照组改变倍数分别为1.01、0.97、1.07、0.99、1.03倍,P均0.05。结论TGF-β诱导细胞内总的ROS表达增加,但过量ROS并非来源于线粒体,机制可能是通过调控细胞内氧化-抗氧化平衡系统的关键酶从而改变细胞内的ROS水平。
[Abstract]:Objective to investigate the effect of transforming growth factor- 尾 (TGF- 尾) on the expression of reactive oxygen species (Ros) in human ovarian cancer cell line SK-OV-3, and to explore its possible mechanism. Methods SK-OV-3 cells in logarithmic growth phase were randomly divided into two groups. The experimental group was cultured with 5 渭 g/mL TGF- 尾 (final concentration was 5 ng / mL) with 2 渭 L serum-free DMEM (the final concentration was 5 ng / mL), and the control group was directly supplemented with 2 mL culture medium. The total reactive oxygen species (Ros) production and mitochondrial origin were detected by flow cytometry, the changes of EMTT in epithelial cells and mesenchymal transformation were detected by real-time fluorescence quantitative PCR, and the mitochondrial respiratory chain complex 鈪，

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