NF-κBp65蛋白及ENA-78在子宫内膜异位症发病中的作用研究

发布时间：2018-06-03 19:02

本文选题：子宫内膜异位症 + 核因子κBp　；参考：《中国实用妇科与产科杂志》2015年07期

【摘要】：目的探讨核因子κBp65(NF-κBp65)蛋白及中性粒细胞活化肽-78(ENA-78)在子宫内膜异位症(内异症)发病中的作用。方法选取2008年10月至2009年2月在山西医科大学第二医院手术切除的卵巢子宫内膜异位囊肿25例,留取异位内膜与在位内膜组织,分为异位内膜组和在位内膜组,选取同期正常的子宫内膜22例为对照组。不同浓度的白细胞介素1β(IL-1β)及IL-1β+二硫氨基甲酸肽吡咯烷(PDTC)分别干预体外传代培养至第3代的在位、异位及正常内膜基质细胞。免疫细胞化学方法及酶联免疫吸附实验(ELISA)检测3组子宫内膜基质细胞NF-κBp65蛋白活化程度及其培养上清液中ENA-78浓度。用IL-1β、IL-1β+PDTC检验各组内膜基质细胞分泌ENA-78及NF-κBp65蛋白的活化程度。结果在位及异位子宫内膜基质细胞培养上清液中ENA-78浓度明显高于正常子宫内膜基质细胞,差异有统计学意义(P0.01);在位及异位子宫内膜基质细胞之间比较,异位子宫内膜基质细胞培养上清液中的ENA-78浓度高于在位子宫内膜基质细胞,差异有统计学意义(P0.01),分别给予IL-1β、IL-1β+PDTC干预后,正常子宫内膜基质细胞上清液中的ENA-78浓度无明显变化(P0.05);而在位及异位内膜基质细胞的上清液中,ENA-78浓度在IL-1β干预后最高,明显高于未干预时及IL-1β+PDTC干预后,差异有统计学意义(P0.01)。3组中,在位及异位内膜基质细胞的NF-κBp65蛋白的活化程度显著高于对照组,差异有统计学意义(P0.05);在位及异位子宫内膜基质细胞NF-κBp65蛋白的活化程度IL-1β干预后最高,显著高于未干预时及IL-1β+PDTC干预后,差异有统计学意义(P0.05)。结论 IL-1β可诱导在位及异位子宫内膜基质细胞NF-κBp65活化,使ENA-78的表达增高。PDTC可抑制此作用,说明NF-κBp65是关键性的调控因子,可能在内异症的发病过程中起到"闸门"的作用,对ENA-78以及其他细胞因子起到重要的调控作用。
[Abstract]:Objective to investigate the role of nuclear factor- 魏 B p65 NF- 魏 Bp65) protein and neutrophil activating peptide -78ENA-78 in the pathogenesis of endometriosis. Methods from October 2008 to February 2009, 25 cases of ovarian endometriosis cysts were surgically resected from the second Hospital of Shanxi Medical University, and the ectopic and eutopic endometrium tissues were collected and divided into ectopic endometrium group and eutopic endometrium group. 22 cases of normal endometrium were selected as control group. Different concentrations of IL-1 尾) and IL-1 尾 dithiocarbamate peptide pyrrolidine were used to interfere with the third generation of eutopic, ectopic and normal endometrial stromal cells. The activation of NF- 魏 Bp65 protein in endometrial stromal cells and the concentration of ENA-78 in the supernatant of endometrial stromal cells were detected by immunocytochemistry and enzyme linked immunosorbent assay (Elisa). The activation of ENA-78 and NF- 魏 Bp65 protein was detected by IL-1 尾 -IL-1 尾 PDTC. Results the concentration of ENA-78 in the supernatant of eutopic and ectopic endometrial stromal cells was significantly higher than that in normal endometrial stromal cells (P 0.01). The concentration of ENA-78 in the culture supernatant of ectopic endometrial stromal cells was higher than that in eutopic endometrial stromal cells (P 0.01). The concentration of ENA-78 in the supernatant of normal endometrial stromal cells did not change significantly (P 0.05), but the concentration of ENA-78 in the supernatant of eutopic and ectopic endometrial stromal cells was the highest after the intervention of IL-1 尾, which was significantly higher than that in the control group and IL-1 尾 PDTC. The activation degree of NF- 魏 Bp65 protein in eutopic and ectopic endometrial stromal cells was significantly higher than that in control group (P 0.05), and the activation degree of NF- 魏 Bp65 protein in eutopic and ectopic endometrial stromal cells was the highest after intervention by IL-1 尾. The difference was significantly higher than that without intervention and after IL-1 尾 PDTC intervention (P 0.05). Conclusion IL-1 尾 can induce the activation of NF- 魏 Bp65 in eutopic and ectopic endometrial stromal cells, and increase the expression of ENA-78. PDTC can inhibit this effect, suggesting that NF- 魏 Bp65 is a key regulatory factor and may play a "gate" role in the pathogenesis of endometriosis. It plays an important role in regulating ENA-78 and other cytokines.
【作者单位】：山西医科大学第二医院妇产科;
【基金】：山西省科技厅基金(20090311059-6)
【分类号】：R711.71

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