miRNA-506对上皮性卵巢癌细胞增殖、侵袭迁移能力的影响研究

发布时间：2018-06-04 20:33

  本文选题：miRNA-506 + 上皮性卵巢癌细胞　； 参考：《暨南大学》2017年硕士论文

【摘要】：目的:探讨miRNA-506对上皮性卵巢癌细胞增殖及侵袭迁移能力的影响方法:1.采用qRT-PCR检测上皮性卵巢癌细胞株A2780、ES-2、Ovar-3和SK-OV-3中miRNA-506的表达水平,筛选出相对高表达及相对低表达miRNA-506的上皮性卵巢癌细胞株。2.利用miRNA-506 inhibitor质粒,转染相对高表达miRNA-506的上皮性卵巢癌细胞株;利用miRNA-506 mimics质粒转染相对低表达miRNA-506的上皮性卵巢癌细胞株。3.采用CCK8检测miRNA-506对上皮性卵巢癌细胞增殖的影响。4.采用Transwell小室方法检测miRNA-506对上皮性卵巢癌细胞侵袭迁移能力的影响。结果:1.上皮性卵巢癌细胞株A2780、ES-2、Ovar-3和SK-OV-3中,细胞系A2780的miRNA-506表达水平相对最高,SK-OV-3相对最低。2.转染miRNA-506 inhibitor质粒的上皮性卵巢癌细胞系A2780的A450值为2.3095±0.0117,阴性转染对照组的A450值为2.2216±0.0228,差异有统计学意义(P0.05);转染miRNA-506 mimics质粒的上皮性卵巢癌细胞系SK-OV-3的A450值为2.0106±0.0950,阴性转染对照组的A450值为2.1744±0.0436,差异有统计学意义(P0.05)。3.转染miRNA-506 inhibitor质粒的上皮性卵巢癌细胞系A2780的迁移细胞数为126±10.91,阴性转染对照组的迁移细胞数为95±11.11,差异有统计学意义(P0.05);转染miRNA-506 mimics质粒的上皮性卵巢癌细胞系SK-OV-3的迁移细胞数为103.2±9.78,阴性转染对照组的迁移细胞数为121.0±5.61,差异有统计学意义(P0.05)。4.转染miRNA-506 inhibitor质粒的上皮性卵巢癌细胞系A2780的侵袭细胞数为:25.0±14.20,阴性转染对照组的侵袭细胞数为16.6±7.89,差异无统计学意义(P0.05);5.转染miRNA-506 mimics质粒的上皮性卵巢癌细胞系SK-OV-3的细胞数为52.4±8.47,其阴性转染对照组的侵袭细胞数为67.2±9.68,差异有统计学意义(P0.05)。结论:1.在上皮性卵巢癌细胞系中,抑制miRNA-506表达后,促进A2780细胞的增殖能力;过表达miRNA-506后,抑制SK-OV-3细胞的增殖能力。表明miRNA-506可抑制上皮性卵巢癌细胞的增殖能力。2.在上皮性卵巢癌细胞系中,抑制miRNA-506表达后,促进A2780细胞迁移能力;过表达miRNA-506后,抑制SK-OV-3细胞迁移能力。表明miRNA-506可抑制上皮性卵巢癌细胞的迁移能力。3.在上皮性卵巢癌细胞系中,抑制miRNA-506表达后,促进A2780细胞侵袭能力;过表达miRNA-506后,抑制SK-OV-3细胞侵袭能力。表明miRNA-506可抑制上皮性卵巢癌细胞的侵袭能力。
[Abstract]:Objective: to investigate the effect of miRNA-506 on proliferation, invasion and migration of epithelial ovarian cancer cells. The expression of miRNA-506 in epithelial ovarian cancer cell line A2780OES-2Ovar-3 and SK-OV-3 was detected by qRT-PCR, and the epithelial ovarian cancer cell line. 2 was screened out with relatively high expression and relatively low expression of miRNA-506. MiRNA-506 inhibitor plasmid was used to transfect epithelial ovarian cancer cell line with relatively high expression of miRNA-506, and miRNA-506 mimics plasmid was used to transfect epithelial ovarian cancer cell line. 3. The effect of miRNA-506 on proliferation of epithelial ovarian cancer cells was detected by CCK8. 4. 4. The effect of miRNA-506 on invasion and migration of epithelial ovarian cancer cells was detected by Transwell chamber method. The result is 1: 1. In epithelial ovarian cancer cell line A2780 / ES-2Ovar-3 and SK-OV-3, the expression of miRNA-506 in A2780 cell line was the highest and the lowest in SK-OV-3 cell line. The A450 value of epithelial ovarian cancer cell line A2780 transfected with miRNA-506 inhibitor plasmid was 2.3095 卤0.0117, the A450 value of negative transfection control group was 2.2216 卤0.0228, the difference was statistically significant (P 0.05), and the A450 value of epithelial ovarian cancer cell line SK-OV-3 transfected with miRNA-506 mimics plasmid was 2.0106 卤0.0950. The A450 value of the radiation group was 2.1744 卤0.0436, and the difference was statistically significant (P 0.05). The number of migration cells of epithelial ovarian cancer cell line A2780 transfected with miRNA-506 inhibitor plasmid was 126 卤10.91, while that of negative control group was 95 卤11.11.The difference was statistically significant (P0.05). The migration of epithelial ovarian cancer cell line SK-OV-3 transfected with miRNA-506 mimics plasmid was fine. The cell number was 103.2 卤9.78, and the number of migration cells in negative transfected control group was 121.0 卤5.61. The difference was statistically significant (P 0.05). The number of invasive cells of epithelial ovarian cancer cell line A2780 transfected with miRNA-506 inhibitor plasmid was 1: 25.0 卤14.20, while that of negative transfection control group was 16.6 卤7.89.The difference was not statistically significant (P 0.05). The number of SK-OV-3 cells transfected with miRNA-506 mimics plasmid was 52.4 卤8.47, while the number of invasive cells in negative transfected control group was 67.2 卤9.68. The difference was statistically significant (P 0.05). Conclusion 1. In epithelial ovarian cancer cell line, inhibition of miRNA-506 expression promoted the proliferation of A2780 cells, and over-expression of miRNA-506 inhibited the proliferation of SK-OV-3 cells. The results showed that miRNA-506 could inhibit the proliferation of epithelial ovarian cancer cells. In epithelial ovarian cancer cell line, inhibition of miRNA-506 expression promoted the migration of A2780 cells, and over-expression of miRNA-506 inhibited the migration of SK-OV-3 cells. It is suggested that miRNA-506 can inhibit the migration ability of epithelial ovarian cancer cells. In epithelial ovarian cancer cell line, inhibition of miRNA-506 expression promoted the invasion ability of A2780 cells, and overexpression of miRNA-506 inhibited the invasion ability of SK-OV-3 cells. These results suggest that miRNA-506 can inhibit the invasion of epithelial ovarian cancer cells.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.31

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