宫腔灌注自体外周血单个核细胞治疗对反复种植失败患者妊娠结局的影响及相关机制

发布时间：2018-06-06 06:42

本文选题：反复种植失败(RIF) + 外周血单个核细胞(PBMCs)　；参考：《中华生殖与避孕杂志》2017年07期

【摘要】：目的探讨宫腔灌注自体外周血单个核细胞(PBMCs)治疗对反复种植失败(RIF)患者妊娠结局的影响及相关机制。方法 36名RIF患者于冻融胚胎移植(FET)前24 h行自体PBMCs宫腔灌注,随访妊娠结局;酶联免疫吸附试验(ELISA)检测宫腔灌注前、后外周血及宫颈分泌物中白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平。结果 RIF患者经宫腔灌注自体PBMCs治疗后得到了较高的临床妊娠率(47.22%);宫颈分泌物中IL-6、IL-6/TNF-α均显著升高(P0.05),TNF-α水平无统计学差异(P0.05),外周血中IL-6、TNF-α水平均无统计学差异(P0.05)。结论宫腔灌注自体PBMCs治疗能提高RIF患者宫腔局部IL-6水平及IL-6/TNF-α比值,有效改善RIF患者临床妊娠率。
[Abstract]:Objective to investigate the effect of intrauterine infusion of autologous peripheral blood mononuclear cells (PBMCs) on pregnancy outcome in patients with recurrent failed implantation. Methods 36 RIF patients were perfused with autologous PBMCs intrauterine cavity 24 hours before embryo transfer into frozen and thawed embryo, and the pregnancy outcome was followed up. The levels of IL-6 and TNF- 伪 in peripheral blood and cervical secretions were observed. Results the clinical pregnancy rate of RIF patients was 47.22% after intrauterine infusion of autologous PBMCs, and the level of IL-6 / TNF- 伪 in cervical secretions was significantly higher than that of P0.05TNF- 伪 level (P0.05A), but there was no significant difference in the level of IL-6TNF- 伪 in peripheral blood. Conclusion Intrauterine infusion of autologous PBMCs can improve the level of intrauterine IL-6 and the ratio of IL-6 / TNF- 伪 in patients with RIF, and improve the clinical pregnancy rate of patients with RIF.
【作者单位】：中南大学湘雅二医院妇产科生殖中心;
【分类号】：R714.8

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