霉菌性阴道炎患者阴道分泌物与外周血T淋巴细胞亚群、细胞因子水平变化及意义

发布时间：2018-06-06 14:47

本文选题：阴道炎 + 霉菌　；参考：《社区医学杂志》2016年24期

【摘要】：目的观察霉菌性阴道炎患者阴道分泌物及外周血T淋巴细胞亚群、细胞因子水平变化,并探讨其临床意义。方法随机选择本院2016年1—12月妇科门诊150例霉菌性阴道炎患者为研究组,同期选择体检健康者150例为对照组。采用流式细胞术和免疫化学发光法分别测定两组患者治疗前后分泌物及外周血T淋巴细胞亚群和Th_1类细胞因子[白细胞介素(interleukin,IL)-2、肿瘤坏死因子(tumor necrosis factor,TNF)-α和干扰素(immune interferon,INF)-γ]、Th_2类细胞因子(IL-4、IL-6和IL-10),比较各组分泌物及血清T淋巴细胞亚群和TH_1/TH_2细胞因子的变化。计量资料比较用t检验,P0.05为差异有统计学意义。结果治疗前,研究组分泌物及外周血T淋巴细胞亚群CD_3~+、CD_4~+、CD_8~+百分比和CD_4~+/CD_8~+,IL-2、TNF-α和INF-γ表达较对照组降低,IL-4、IL-6和IL-10表达较对照组升高(均P0.05);治疗后,两组以上指标比较差异均无统计学意义(均P0.05)。结论霉菌性阴道炎患者存在Th_1/Th_2失衡,表现为Th_2淋巴细胞亚群占优势的免疫应答,Th_1类介导的IL-2、TNF-α和INF-γ低表达,Th_2类介导的IL-4、IL-6和IL-10高表达,Th_1/Th_2细胞介导的细胞免疫与霉菌性阴道炎患者的免疫功能失衡有关。
[Abstract]:Objective to observe the changes of T lymphocyte subsets and cytokines in vaginal secretions and peripheral blood of patients with fungal vaginitis and to explore its clinical significance. Methods 150 patients with fungal vaginitis in gynecological outpatient department from January to December 2016 were randomly selected as study group and 150 healthy persons as control group. The levels of T lymphocyte subsets and Th1 cytokines in secretions and peripheral blood before and after treatment were determined by flow cytometry and immunochemiluminescence, respectively. The changes of T lymphocyte subsets and T lymphocyte subsets in secretions and serum and 1 / 1 / T T _ 2 cytokines were compared between IL-4 and IL-10 in each group. There was statistical significance in the comparison of measurement data with t test (P 0.05). Results before treatment, the expression of CD3 CD4 CD4 CD8 ~% and CD4 / CD8 + IL-2TNF- 伪 and INF- 纬 in secretions and peripheral blood T lymphocyte subsets of the study group were lower than those in the control group (all P 0.05), and the expression of TNF- 伪 and INF- 纬 in the study group was significantly lower than that in the control group (all P 0.05), and after treatment, the expression of TNF- 伪 and INF- 纬 in the study group was significantly lower than that in the control group. There was no significant difference between the two groups (P 0.05). Conclusion the patients with mildew vaginitis have 1 / 1 / Th2 / Th2 imbalance. The low expression of IL-2TNF- 伪 and INF- 纬 mediated by Th2 type T NF- 伪 and INF- 纬, and the high expression of IL-4, IL-6 and IL-10 mediated by Th _ 2 lymphocyte subsets were related to the imbalance of immune function in patients with fungal vaginitis.
【作者单位】：深圳市宝安区中心医院检验科;深圳市宝安区西乡预防保健所门诊部;深圳市麒麟山疗养院;深圳市宝安区中心医院社区健康服务管理中心;
【基金】：2015年度深圳市宝安区科技计划项目(2015156)
【分类号】：R711.31;R446.1

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