子宫内膜异位症不孕女性腹腔液菌群构成及TNF-α与IL-37水平研究

发布时间：2018-06-06 18:44

  本文选题：子宫内膜异位症 + 不孕症　； 参考：《中国妇产科临床杂志》2015年05期

【摘要】：目的探讨子宫内膜异位症(endometriosis,EMs)不孕患者腹腔液中的微生物菌群及其肿瘤坏死因子α(TNF-α)和白介素37(IL-37)水平的变化。方法采集内膜异位症不孕组(T)29例和对照组(C)19例女性的腹腔液(peritoneal fluid,PF)标本,分别进行基因组DNA提取后Ion Torrent PGM平台测序检测微生物菌群和酶联免疫吸附试验(ELISA)检测IL-37及TNF-α水平。结果 EMs不孕患者和对照组女性PF中均发现了多种菌群存在,在菌门水平上主要分为变形菌门Proteobacteria、厚壁菌门Firmicutes、拟杆菌门Bacteriodetes、放线菌门Actinobacteria、梭杆菌门Fusobacteria,其中Proteobacteria为主要菌群,占60%以上的序列,软壁菌门Tenericutes只在对照组中检出1例,各菌门在两组间差异无统计学意义(P0.05)。EMs不孕妇女腹腔液中TNF-a和IL-37水平分别为(75.87±8.47)pg/ml、(372.03±68.02)pg/ml,高于对照组(57.76±8.29)pg/ml和(261.37±52.78)pg/ml(P0.05);EMs不孕组Ⅲ~Ⅳ期TNF-a的水平为(83.47±4.73)pg/ml,显著高于Ⅰ~Ⅱ期(74.29±8.26)pg/ml(P0.05),IL-37的水平在二者中差异无统计学意义(P0.05);内膜异位症不孕组中两者存在正相关关系(r=0.569,P0.001),对照组中两者不存在相关关系(r=-0.247,P=0.522)。结论 EMs不孕患者和对照组女性腹腔液中存在多种菌群,EMs不孕组中TNF-a和IL-37含量增高可能在EMs不孕症的发生机制中起到重要作用。
[Abstract]:Objective to investigate the changes of microflora, tumor necrosis factor 伪 (TNF- 伪) and interleukin-37 (IL-37) in peritoneal fluid of infertile patients with endometriosis. Methods the peritoneal fluid of 29 infertile women with endometriosis and 19 female controls with endometriosis were collected, and the levels of IL-37 and TNF- 伪 were detected by Ion Torrent PGM platform sequencing after genomic DNA extraction. The levels of IL-37 and TNF- 伪 were detected by enzyme linked immunosorbent assay (Elisa). Results A variety of microflora was found in female PF of infertile patients and control group, which was divided into Proteobacteria, Firmicutes, Bacteriodetes, Actinobacteria, Fusobacteria, Proteobacteria, among which Proteobacteria was the main group. Tenericutes was detected only in 1 case in the control group. There was no significant difference between the two groups in the levels of TNF-a and IL-37 in the peritoneal fluid of infertile women (75.87 卤8.47 pg / ml, 372.03 卤68.02), which were higher than those in the control group (57.76 卤8.29)pg/ml) and the control group (261.37 卤52.78). The levels of TNF-a in stage 鈪，

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