O-GLcNAc糖基化介导的β-catenin水平变化对卵巢癌细胞增殖的影响

发布时间：2018-06-06 20:17

本文选题：卵巢癌 + O-GlcNAc　；参考：《现代妇产科进展》2017年07期

【摘要】：目的:探讨O-GLcNAc糖基化修饰对卵巢癌细胞内β-catenin水平以及细胞增殖的影响。方法:利用慢病毒转染技术构建O-GLcNAc低表达SKOV3细胞模型。利用O-GLcNAc水解酶(OGA)抑制剂PUGNAc构建高表达A2780细胞模型。流式细胞技术检测构建O-GLcNAc糖基化对细胞周期的影响。MTT法检测O-GLcNAc糖基化对细胞增殖的影响。Westernblot法检测不同细胞模型内β-catenin表达情况。CO-IP法检测不同细胞模型内β-catenin的糖基化修饰水平。结果:下调细胞内O-GLcNAc糖基化水平能抑制细胞的增殖能力,上调细胞内O-GLcNAc糖基化水平能增强细胞的增殖能力。下调细胞内O-GLcNAc糖基化水平后,β-catenin表达以及糖基化修饰水平降低;上调细胞内O-GLcNAc糖基化水平后,β-catenin表达以及糖基化修饰水平增高。结论:卵巢癌细胞内OGLcNAc糖基化水平变化能通过β-catenin对细胞增殖产生影响。
[Abstract]:Aim: to investigate the effect of O-GLcNAc glycosylation on 尾 -catenin level and cell proliferation in ovarian cancer cells. Methods: O-GLcNAc low expression SKOV3 cell model was constructed by lentivirus transfection technique. The high expression A2780 cell model was constructed by using O-GLcNAc hydrolase (OGA) inhibitor PUGNAc. Flow cytometry was used to detect the effect of glycosylation of O-GLcNAc on cell cycle. MTT assay was used to detect the effect of O-GLcNAc glycosylation on cell proliferation. Western blot was used to detect the expression of 尾 -catenin in different cell models. CO-IP method was used to detect the glycosylation modification level of 尾 -catenin in different cell models. Results: down-regulating the level of O-GLcNAc glycosylation could inhibit the proliferation of cells, and up-regulate the level of O-GLcNAc glycosylation could enhance the proliferation of cells. After down-regulating the glycosylation level of O-GLcNAc, the expression of 尾 -catenin and the level of glycation modification decreased, but the expression of 尾 -catenin and the level of glycosylation increased after up-regulating the level of glycosylation of O-GLcNAc. Conclusion: the change of OGLcNAc glycosylation level in ovarian cancer cells can affect cell proliferation through 尾-catenin.
【作者单位】：第四军医大学西京医院妇产科;宁夏军区医院妇产科;宁夏医科大学;
【分类号】：R737.31

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