正常宫颈、宫颈上皮内瘤变Ⅲ级和宫颈癌的拉曼光谱分析

发布时间：2018-06-07 04:33

本文选题：拉曼光谱技术 + 宫颈癌　；参考：《吉林大学》2014年硕士论文

【摘要】：目的：(1)采用拉曼光谱仪对正常宫颈、CIN III和宫颈癌进行光谱分析比较，总结各自的特征峰，对其进行分析总结；(2)拉曼光谱技术对宫颈癌、CIN III组织的光谱分析是否可用于临床筛查，并成为宫颈癌及癌前病变筛查的一种新方法。材料和方法：组织标本取自于2013年4月—2013年11月在吉林大学第一医院妇科行手术治疗的患者，共63例。其中，宫颈鳞状细胞癌组织标本33例、CIN III组织标本10例为实验组，正常宫颈组织标本20例为对照组，采用拉曼光谱仪对正常宫颈组织、CIN III组织和宫颈癌组织进行光谱分析，得出各自的拉曼图谱，并对其分析。结果：1、在不同组织中拉曼光谱的特征峰的位置不同，具体表现：正常宫颈组织主要的拉曼光谱特征峰在640、853、928、1005、1242、1450、1660-1cm；CIN III组织主要的拉曼光谱特征峰在853、931、1003、1242、1341、1447、1657-1cm；宫颈癌主要的拉曼光谱特征峰在642、853、1003、1345、1443、1657-1cm。 2、拉曼光谱在不同组织中同一特征峰的相对强度不同：以核酸为主要特征峰的1345cm-1处，核酸含量差异较大，依次为宫颈癌组织＞CIN III组织＞正常宫颈组织（P＜0.01）；以蛋白质为主要特征峰的1003cm-1处，，蛋白质含量差异较大，依次为宫颈癌＞CIN III组织＞正常宫颈组织（P＜0.01）；以脂类为特征峰的1443cm-1处，正常组织的相对强度高于CIN III组织和宫颈癌的相对强度，但三者的相对强度无明显差异（P＞0.05）。结论：1、拉曼光谱在正常宫颈组织、CIN III、宫颈癌组织中其特征峰的位置不同。2、拉曼光谱在正常宫颈组织、CIN III、宫颈癌组织中同一特征峰的相对强度不同。3、拉曼光谱技术有望成为筛查宫颈癌和宫颈癌前病变的检测方法。
[Abstract]:Objective: to analyze and compare the spectra of cin III and cervical cancer by Raman spectrometer, summarize their characteristic peaks and sum up their characteristic peaks. Whether Raman spectroscopy can be used for clinical screening of cin III tissue from cervical cancer. It has become a new method for screening cervical cancer and precancerous lesions. Materials and methods: tissue specimens were collected from 63 patients who underwent gynecologic surgery in the first Hospital of Jilin University from April 2013 to November 2013. Among them, 33 specimens of cervical squamous cell carcinoma (SCC) and 10 specimens of cin III (n = 10) were used as experimental group and 20 cases as control group. Raman spectrometer was used to analyze cin III tissue and cervical carcinoma tissue of normal cervix. The Raman spectra were obtained and analyzed. Results the characteristic peaks of Raman spectra in different tissues were different in different tissues. The main Raman characteristic peaks of normal cervix were 640853928 / 1005 / 1242n ~ 1450 ~ 1660-1cm ~ (-1). The main characteristic peaks of Raman spectra of cin / III were 853931 / 100312441 / 14447 / 1657-1 cm and 642853313445 / 14431657-1cm / cm, respectively, and the main characteristic peaks of Raman spectra of cervical cancer were 64285313455 / 14431657-1cm respectively, and the main characteristic peaks of Raman spectra of cervical cancer were 64285313455 ~ 14431657-1cm ~ (-1) cm. 2. The relative intensity of the same characteristic peak in different tissues is different: the nucleic acid content is different at the 1345cm-1 where nucleic acid is the main characteristic peak. The order was cervical cancer > CIN III > normal cervix (P < 0.01), the content of protein in 1003cm-1 with protein as the main characteristic peak was higher than that in normal cervix (P < 0.01), and that in 1443cm-1 with lipid as the characteristic peak was higher than that in normal cervix (P < 0.01), the order was cervical cancer > CIN III > normal cervix (P < 0.01). The relative strength of normal tissue was higher than that of CIN III and cervical cancer, but there was no significant difference among them (P > 0.05). Conclusion in normal cervical tissue, the Raman spectrum has different position of characteristic peak in cervical cancer tissue, Raman spectrum in normal cervix tissue of cin III, Raman spectrum in normal cervix tissue of cin III, and the relative intensity of the same characteristic peak in cervical cancer tissue is different. It is expected to be a screening method for cervical cancer and cervical precancerous lesions.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

【参考文献】