宫颈癌组织中Ang-2及其受体Tie-2的表达变化

发布时间：2018-06-07 04:56

本文选题：宫颈肿瘤宫颈癌 + 血管生成素-　；参考：《山东医药》2017年35期

【摘要】：目的观察宫颈癌组织中血管生成素-2(Ang-2)和其受体酪氨酸激酶受体2(Tie-2)表达变化,并探讨其临床意义。方法选择2014年1月~2017年1月收治的86例宫颈癌患者的手术切除宫颈癌组织标本为观察组,同期收治的86例子宫肌瘤患者的正常宫颈组织标本为对照组。采用免疫组化法定性检测两组Ang-2、Tie-2蛋白表达情况。采用RT-PCR法定量检测两组Ang-2、Tie-2 mRNA表达量,采用Western blotting法定量检测两组Ang-2、Tie-2蛋白表达量。分析宫颈癌组织Ang-2、Tie-2蛋白定性表达情况与宫颈癌临床病理参数的关系。结果观察组Ang-2、Tie-2 mRNA及蛋白表达量均高于对照组(P均0.05)。免疫组化染色结果观察组Ang-2表达阳性51例(59.30%)、Tie-2表达阳性54例(62.79%),对照组Ang-2表达阳性35例(40.70%)、Tie-2表达阳性37例(43.02%),观察组Ang-2、Tie-2蛋白阳性表达率均高于对照组(P均0.05)。宫颈癌组织Ang-2、Tie-2阳性表达与宫颈癌FIGO分期、淋巴结转移有关(P均0.05),与年龄、组织学类型、分化程度无关(P均0.05)。结论宫颈癌组织Ang-2和其受体Tie-2表达上调。检测宫颈癌组织Ang-2和其受体Tie-2表达有助于判断宫颈癌患者的FIGO分期和淋巴结转移情况。
[Abstract]:Objective to observe the expression of angiopoietin-2 Ang-2) and its receptor tyrosine kinase receptor 2-Tie-2 in cervical carcinoma and to explore its clinical significance. Methods 86 cases of cervical carcinoma treated from January 2014 to January 2017 were selected as observation group and 86 cases of normal cervical tissue as control group. The expression of Ang-2 tie-2 protein was detected qualitatively by immunohistochemical method. The expression of Ang-2 tie-2 mRNA in two groups was detected by RT-PCR assay, and the expression of Ang-2 tie-2 protein was detected by Western blotting assay. To analyze the relationship between the qualitative expression of Ang-2 tie-2 protein and the clinicopathological parameters of cervical carcinoma. Results the expression of mRNA and protein of Ang-2 tie-2 in the observation group was higher than that in the control group (P 0.05). The results of immunohistochemical staining showed that there were 51 cases of positive expression of Ang-2 in observation group and 54 cases of positive expression of Tie-2 in 59.30 cases, while 35 cases of positive expression of Ang-2 in control group were positive in 37 cases (43.022%). The positive rate of Ang-2 tie-2 protein in observation group was higher than that in control group (P 0.05). The positive expression of Ang-2pTie-2 in cervical carcinoma was correlated with FIGO stage, lymph node metastasis (P < 0.05), and had no correlation with age, histological type and differentiation degree (P < 0.05). Conclusion the expression of Ang-2 and its receptor Tie-2 is up-regulated in cervical carcinoma. The expression of Ang-2 and its receptor Tie-2 in cervical carcinoma is helpful to judge the FIGO stage and lymph node metastasis.
【作者单位】：盐城市第一人民医院;
【基金】：江苏省科技计划项目(BK2015113)
【分类号】：R737.33

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