辅助生殖技术对人胎盘DNA甲基化水平的影响
发布时间：2018-06-08 07:18
本文选题：辅助生殖技术(ART) + 胎盘 ； 参考：《中华生殖与避孕杂志》2017年11期
【摘要】：目的探究辅助生殖技术(ART)助孕对单胎足月分娩新生儿的胎盘及胎盘总DNA甲基化水平和甲基化相关基因表达的影响。方法选择2016年6月—2016年12月期间单胎足月分娩的患者,ART子代为ART组,自然妊娠子代为对照组,两组按孕妇年龄、孕周等进行配对后,比较两组胎盘质量、新生儿体质量、胎盘效率。采用酶联免疫吸附测定法(ELISA)测定胎盘总DNA的甲基化水平,采用实时定量聚合酶链式反应(RT-q PCR)方法检测胎盘甲基转移酶(DNA methyltransferase,DNMT)家族和去甲基化酶(ten-eleven translocation,TET)家族的m RNA表达水平。结果 (1)ART组新生儿体质量[(3 650.50±393.84)g]、胎盘重量[(633.05±98.58)g]显著增加,且高于对照组[(3 339.50±377.01)g,(563.00±85.60)g](P0.05)。(2)ART组胎盘总DNA甲基化水平(1.22%±0.45%)显著低于对照组(2.08%±0.98%)(P0.05)。(3)ART组DNMT3B、TET1、TET2、TET3的m RNA相对表达量分别为4.61±1.06、4.66±1.06、4.99±1.09、4.92±1.13,显著高于对照组(1.23±0.55、1.27±0.56、1.47±0.50、1.41±0.61)(P0.05),DNMT1、DNMT3A的表达水平无统计学差异(P0.05)。结论 ART会导致人类妊娠晚期胎盘过度生长,总DNA甲基化水平降低,DNMT3B、TET1、TET2、TET3表达上调可能是导致胎盘DNA甲基化水平降低的机制。
[Abstract]:Objective to investigate the effect of assisted reproductive technique (ART) on the total DNA methylation level and methylation-related gene expression in placenta and placenta of newborns with single term delivery. Methods from June 2016 to December 2016, patients with single term delivery were selected as art group and natural pregnancy as control group. The two groups were matched according to the age and gestational weeks of pregnant women, and the placental weight and body weight of newborns were compared between the two groups. Placental efficiency. The methylation level of total placental DNA was determined by Elisa, and the mRNA expression of methyltransferase DNA methyltransferase (DNMT) family and ten-eleven translocation (TETT) family were detected by real-time quantitative polymerase chain reaction (RT-q PCR) method. Results the body weight [3 650.50 卤393.84 g] and placental weight [633.05 卤98.58 g] were significantly increased in the art group. 涓旈珮浜庡鐓х粍[(3 339.50卤377.01)g,(563.00卤85.60)g](P0.05).(2)ART缁勮儙鐩樻，
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