应用iTRAQ技术筛选先天性心脏畸形胎儿母亲血清差异表达蛋白

发布时间：2018-06-08 13:37

本文选题：同位素标记相对和绝对定量技术 + 先天性心脏畸形　；参考：《中国医科大学学报》2017年06期

【摘要】：目的筛选先天性心脏畸形(CHD)母体血清差异表达蛋白质,明确和CHD相关的候选标志物。方法收集40例CHD胎儿(实验组)母亲血清和10例正常胎儿(对照组)母亲血清,其中实验组包括法洛氏四联征10例,室间隔缺损10例,共同动脉干10例,其他少见先天性心脏畸形10例。每组10例标本等体积混合后,应用同位素标记相对和绝对定量技术(iTRAQ)对蛋白质进行鉴定和相对定量。结果孕妇外周血血清中共鉴定到蛋白质606个,实验组与对照组差异达到1.5倍以上的蛋白质47个,其中23个在实验组中上调,24个在实验组中下调。结论基于i TRAQ技术的蛋白质组学方法能鉴定出多种CHD相关的差异蛋白,CHD发生是一个多种蛋白质分子参与的结果。
[Abstract]:Objective to screen differentially expressed proteins in maternal serum of congenital heart malformation (CHD) and to identify candidate markers associated with CHD. Methods the maternal serum of 40 CHD fetuses (experimental group) and 10 normal fetuses (control group) were collected, including 10 cases of Fallot's tetralogy, 10 cases of ventricular septal defect and 10 cases of common artery trunk. Other rare congenital heart malformations in 10 cases. 10 samples of each group were mixed in the same volume, then the protein was identified and quantified by using the relative and absolute quantitative techniques of isotopic labeling. Results A total of 606 proteins were identified in maternal peripheral blood serum. 47 proteins with a difference of more than 1.5 times were identified in the experimental group and the control group, 23 of which were up-regulated in the experimental group and 24 were down-regulated in the experimental group. Conclusion the proteomics method based on I TRAQ technique can identify a variety of CHD related differential proteins CHD is a result of a variety of protein molecules involved.
【作者单位】：中国医科大学附属盛京医院超声科;中国医科大学附属盛京医院国家卫生和计划生育委员会小儿先天畸形重点实验室;中国医科大学附属盛京医院泌尿外科;
【基金】：国家自然科学基金青年基金项目(81600258);国家自然科学基金(81671469) 国家重点研发计划(2016YFC1000505)
【分类号】：R445.1;R714.5

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