β-catenin、Wnt3a和Lgr5在不同宫颈组织中的表达及其相关性研究

发布时间：2018-06-10 16:40

本文选题：宫颈癌 + Wnt信号通路　；参考：《皖南医学院》2017年硕士论文

【摘要】：目的:检测β-catenin、Wnt3a和Lgr5在不同宫颈组织中的表达情况,并分析三者之间表达相关性,探讨三者与宫颈癌发生发展的关系。方法:采用免疫组织化学法(SP法)检测不同宫颈组织标本中β-catenin、Wnt3a和Lgr5的表达,包括宫颈癌组织61例,宫颈上皮内瘤变组织30例(CINI12例,CINII-III 18例),正常宫颈组织19例.结果:1.β-catenin正常情况下为连续的包膜表达,病理状态时,则出现胞膜表达的缺失,胞质/核内出现不同程度的异常表达。β-catenin异常表达率在正常宫颈组织组5.26%(1/19),CINI组33.33%(4/12),CINII-III组38.89%(7/18),CC组75.41%(46/61),4组比较(χ_2=32.958,P0.05),有统计学意义;在CC组G1、G2、G3各级的异常表达率分别为41.67%(5/12)、76.67%(23/30)、94.74%(18/19),可以看出在G3组表达突出(χ_2=11.221,P0.05)有统计学意义;在浅浸润组和深浸润组异常表达率分别为55.56%(10/18)和83.72%(36/43)(χ_2=5.428,P0.05),有统计学意义。而其异常表达与临床分期、年龄、组织学类型、年龄可能无关(P0.05)。2.Wnt3a阳性表达率在正常宫颈组织组10.53%(2/19),CINI组41.67%(5/12),CINII-III组55.56%(10/18),CC组40.98%(25/61),4组比较(χ_2=8.723,P0.05),有统计学意义;在淋巴结转移组为59.09%(13/22),无淋巴结转移组30.78%(12/39),(χ_2=4.665,P0.05),有统计学意义。而Wnt3a在临床分期、病理分级、浸润深浅、组织学类型、年龄的阳性表达可能无关(P0.05)。3.Lgr5阳性表达率在正常宫颈组织组0%(0/19),CINI组25%(3/12),CINII-III组38.89%(7/18),CC组65.57%(40/61),4组比较(χ_2=28.130,P0.05),有统计学意义。Lgr5在宫颈癌G1 33.33%(4/12)、G2 70%(21/30)、G3 78.95%(15/19)各级的阳性表达有统计学意义(χ_2=7.291);在浅浸润组和深浸润组异常表达率分别为38.89%(7/18)和76.74%(33/43)(χ_2=6.149,P0.05),也有统计学意义。而Lgr5在临床分期、病理分级、浸润深浅、组织学类型、年龄的阳性表达可能无关(P0.05)。4.在β-catenin和Wnt3a在CC组中共阳性表达23例,共阴性表达13例,两者之间表达呈正相关(r=0.321);β-catenin和Lgr5在CC组共阳性表达35例,共阴性表达10例,两者之间表达呈正相关(r=0.387);Wnt3a和Lgr5在CC组共阳性表达13例,共阴性表达9例,两者之间表达无统计学意义(P0.05)。结论:1.β-catenin、Wnt3a和Lgr5在正常宫颈组织、CINI、CINII-III及CC中,β-catenin的异常表达、Wnt 3a和Lgr5的阳性表达均呈逐渐上升趋势,提示β-catenin、Wnt3a和Lgr5可能影响宫颈癌的发生发展。2.Wn3a可能与宫颈癌前病变的发生及宫颈癌的转移有关,β-catenin和Lgr5可能与宫颈癌的恶性程度及侵袭性有关3.Lgr5与β-catenin、Wnt 3a与β-catenin的表达具有一致性,呈正相关,提示Lgr5与Wnt 3a的表达可能激活了wnt信号通路,导致了β-catenin异位至胞质/核内,进而引起细胞异常增生及宫颈癌的发生。
[Abstract]:Objective: to detect the expression of 尾 -cateninine Wnt3a and Lgr5 in different cervical tissues, and to analyze the relationship between the expression of Wnt3a and Lgr5, and to explore the relationship between the expression of Wnt3a and Lgr5 in the occurrence and development of cervical cancer. Methods: immunohistochemical staining was used to detect the expression of 尾 -cateninine Wnt3a and Lgr5 in different cervical tissues, including 61 cases of cervical carcinoma, 30 cases of cervical intraepithelial neoplasia, 12 cases of CINII-III and 19 cases of normal cervical tissue. Results: 1. 尾 -catenin was expressed in a continuous capsule under normal conditions, but the cell membrane expression was absent in pathological state. The abnormal expression rate of 尾 -catenin in normal cervix tissue group was 5.26 / 119% in normal cervix tissue group and 33.3333 / 12% in CINI group and 38.89% / 12% in CINII-III group (P < 0.05). In CC group, the abnormal expression rates of G1G2G3 were 41.67% and 51.67%, respectively. There were significant differences in abnormal expression in G3 group (蠂 211.221 P 0.05), in shallow infiltrating group and deep infiltrating group were 55.56% 10 / 18 and 83.722% 36 / 43 (蠂 2 / 25.428 P 0.05 P 0.05, respectively). However, the abnormal expression of CINII-III was not correlated with clinical stage, age, histological type and age. 2. The positive rate of Wnt3a expression in normal cervix tissue group was 10.53 / 20% CINI group (41.67%) and CINII-III group (55.56% / 18CC group, 40.98% 25 / 61%) group (蠂 ~ 2T = 8.723 P 0.05). In the lymph node metastasis group, it was 59.09 / 13 / 22, and in the non-lymph node metastasis group 30.78 / 39 / 39 (蠂 ~ 24.665 / P0.05), which was statistically significant. And Wnt3a in clinical stage, pathological grade, invasion depth, histological type, The positive expression of age may not be related to the positive rate of P0.05. 3. The positive expression rate of Lgr5 in normal cervix tissue group was 38.89% CINII-III and 65.5728.130% P0.05 in normal cervical tissue group (蠂 228.130P0.05). There was statistical significance at all levels (蠂 228.130P 0.05). There was statistical significance at all levels (蠂 228.130P0.05, P 0.05). There was statistical significance at all levels (蠂 228.130P0.05, P 0.05). There was statistical significance at all levels (蠂 228.130P0.05, P 0.05). There was statistical significance at all levels of G3 78.951519 in normal cervical tissue group (蠂 228.130P0.05) (蠂 228.130P 0.05). There was statistical significance at all levels (蠂 228.130P 0.05, P 0.05) in CC group (蠂 228.130P 0.05). The abnormal expression rates were 38.89 / 18 and 76.74 / 43 respectively in the deep infiltrating group (蠂 2 + 6.149% P 0.05), which were also statistically significant. However, the positive expression of Lgr5 in clinical stage, pathological grade, depth of invasion, histological type and age may not be related to P0.05. 4. The positive expression of 尾 -catenin and Wnt3a was positive in 23 cases and negative expression in 13 cases in CC group, and the positive expression of 尾 -catenin and Lgr5 was positive in 35 cases and negative expression in 10 cases in CC group, the positive expression of 尾 -catenin and Wnt3a in CC group was positively correlated with that of 尾 -catenin and Wnt3a in CC group. The positive expression of Wnt3a and Lgr5 in CC group was positive in 13 cases and negative in 9 cases. There was no significant difference in the expression of Wnt3a and Lgr5 between the two groups. Conclusion the abnormal expression of 尾 -catenin Wnt3a and Lgr5 in CINII-III and CC of normal cervix tissues showed an increasing tendency of Wnt3a and Lgr5. These results suggest that 尾 -cateninine Wnt3a and Lgr5 may affect the occurrence and development of cervical cancer. 2. Wn3a may be related to the occurrence of precancerous lesions and metastasis of cervical cancer, 尾 -catenin and Lgr5 may be related to the malignancy and invasiveness of cervical carcinoma 3.The expression of Lgr5 and 尾 -catenin WNT 3a and 尾 -catenin may be consistent. The positive correlation suggested that the expression of Lgr5 and Wnt 3a might activate the wnt signaling pathway, leading to 尾 -catenin ectopic into cytoplasm / nucleus, and then to the abnormal proliferation of cells and the occurrence of cervical cancer.
【学位授予单位】：皖南医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.33

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