孕妇外周血中ERG基因甲基化状态及定量研究

发布时间：2018-06-15 02:53

本文选题：ERG基因 + 甲基化　；参考：《现代妇产科进展》2015年06期

【摘要】：目的:探讨ERG基因作为孕妇外周血中胎儿特异性标记物的可行性。方法:随机选取健康未妊娠妇女5例、正常妊娠孕妇65例、产后3天妇女20例的外周血样本,以及早期妊娠行人流的绒毛组织样本10例。应用甲基化敏感限制性内切酶PCR(MSRE-PCR)及荧光定量PCR方法检测样本中ERG基因甲基化状态,并进行相对定量分析。结果:(1)健康未妊娠妇女、顺产后3天妇女外周血浆及孕妇血细胞中均未检出甲基化的ERG基因;(2)绒毛组织样本中甲基化的ERG基因检出率100%;(3)正常妊娠孕妇外周血浆中甲基化ERG基因检出率为76.9%,中期、晚期妊娠ERG基因的含量分别是早期妊娠的3.18倍和5.46倍。结论:甲基化的ERG基因是可靠的胎儿特异性标记;正常妊娠孕妇的外周血浆中甲基化的ERG基因的检出量随孕周增加而增加。
[Abstract]:Objective: To investigate the feasibility of the ERG gene as a fetal specific marker in the peripheral blood of pregnant women. Methods: 5 healthy non pregnant women, 65 normal pregnant women, 20 women's peripheral blood samples from 3 day postpartum women, and 10 samples of the villi tissue in the early pregnancy, and 10 cases of the early pregnancy human villus tissue, were used to apply methylation sensitive restriction endonuclease PCR (MSRE-PCR). The methylation status of ERG gene in the samples was detected by the fluorescence quantitative PCR method and relative quantitative analysis was carried out. Results: (1) no methylation ERG gene was detected in the peripheral plasma and pregnant women's blood cells for 3 days after the birth of healthy unpregnant women; (2) the detection rate of methylation ERG gene in the villous tissue samples was 100%; (3) the peripheral blood of pregnant women of normal pregnancy was (3) The detection rate of methylated ERG gene in the pulp was 76.9%, and the content of ERG gene in the middle stage was 3.18 times and 5.46 times of the early pregnancy respectively. Conclusion: the methylation ERG gene is a reliable fetal specific marker, and the detection of methylation of ERG gene in the peripheral plasma of normal pregnant women increases with the increase of gestational age.
【作者单位】：西安交通大学医学院第二附属医院超声医学科;西安交通大学医学院第二附属医院妇产科;
【基金】：2015年陕西省社发攻关项目(No:2015SF126) 院重大科研方向科研基金资助[No:YJ(ZDJH)201303]
【分类号】：R714.55

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